Academic Journal
Search and processing of Holliday junctions within long DNA by junction-resolving enzymes
| العنوان: | Search and processing of Holliday junctions within long DNA by junction-resolving enzymes |
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| المؤلفون: | Kaczmarczyk, AP, Déclais, A-C, Newton, MD, Boulton, SJ, Lilley, DMJ, Rueda, DS |
| المساهمون: | Medical Research Council, Wellcome Trust |
| المصدر: | 13 ; 1 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2022 |
| المجموعة: | Imperial College London: Spiral |
| الوصف: | Resolution of Holliday junctions is a critical intermediate step of homologous recombination in which junctions are processed by junction-resolving endonucleases. Although binding and cleavage are well understood, the question remains how the enzymes locate their substrate within long duplex DNA. Here we track fluorescent dimers of endonuclease I on DNA, presenting the complete single-molecule reaction trajectory for a junction-resolving enzyme finding and cleaving a Holliday junction. We show that the enzyme binds remotely to dsDNA and then undergoes 1D diffusion. Upon encountering a four-way junction, a catalytically-impaired mutant remains bound at that point. An active enzyme, however, cleaves the junction after a few seconds. Quantitative analysis provides a comprehensive description of the facilitated diffusion mechanism. We show that the eukaryotic junction-resolving enzyme GEN1 also undergoes facilitated diffusion on dsDNA until it becomes located at a junction, so that the general resolution trajectory is probably applicable to many junction resolving enzymes. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | Nature Communications; http://hdl.handle.net/10044/1/100020; MC_UP_1102/5; 206292/C/17/Z |
| DOI: | 10.1038/s41467-022-33503-6 |
| الاتاحة: | http://hdl.handle.net/10044/1/100020 https://doi.org/10.1038/s41467-022-33503-6 |
| Rights: | © The Author(s) 2022. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. ; http://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.B4CE2A49 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41467-022-33503-6 |
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