Academic Journal
Studies of the conformation-dependent neutralizing epitopes of simian immunodeficiency virus envelope protein
| العنوان: | Studies of the conformation-dependent neutralizing epitopes of simian immunodeficiency virus envelope protein |
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| المؤلفون: | Javaherian, K, Langlois, A J, Montefiori, D C, Kent, K A, Ryan, K A, Wyman, P D, Stott, J, Bolognesi, D P, Murphey-Corb, M, Larosa, G J |
| المصدر: | Journal of Virology ; volume 68, issue 4, page 2624-2631 ; ISSN 0022-538X 1098-5514 |
| بيانات النشر: | American Society for Microbiology |
| سنة النشر: | 1994 |
| الوصف: | It has been shown previously that the major neutralizing epitopes in simian immunodeficiency virus (SIV) are discontinuous and conformation dependent and that the V3 loop, in contrast to that of human immunodeficiency virus (HIV) type 1, does not by itself elicit neutralizing antibodies (K. Javaherian et al., Proc. Natl. Acad. Sci. USA 89:1418-1422, 1992). We now present data showing that on the basis of fractionation of infected macaque sera, protease digestion of the envelope, and binding properties of two neutralizing monoclonal antibodies to SIV and SIV-HIV chimeric envelope proteins, changes in V3 can disrupt the conformation-dependent neutralization region. The chimeric protein did not produce significant neutralizing antibodies against either SIV or HIV. We also report that neutralizing antibodies elicited by recombinant SIV envelope proteins of mac251 and B670 isolates cross-neutralize. Finally, we show that deglycosylation of the SIV envelope results in a molecule which binds neither soluble CD4 nor the neutralizing monoclonal antibodies being investigated here and does not elicit sera with a significant neutralizing titer. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1128/jvi.68.4.2624-2631.1994 |
| الاتاحة: | http://dx.doi.org/10.1128/jvi.68.4.2624-2631.1994 https://journals.asm.org/doi/pdf/10.1128/jvi.68.4.2624-2631.1994 |
| Rights: | https://journals.asm.org/non-commercial-tdm-license |
| رقم الانضمام: | edsbas.B4AC1501 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1128/jvi.68.4.2624-2631.1994 |
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