Academic Journal
Tailoring the resolution of single-cell RNA sequencing for primary cytotoxic T cells.
| العنوان: | Tailoring the resolution of single-cell RNA sequencing for primary cytotoxic T cells. |
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| المؤلفون: | Kanev, K., Roelli, P., Wu, M., Wurmser, C., Delorenzi, M., Pfaffl, M.W., Zehn, D. |
| المصدر: | Nature communications, vol. 12, no. 1, pp. 569 |
| سنة النشر: | 2021 |
| المجموعة: | Université de Lausanne (UNIL): Serval - Serveur académique lausannois |
| مصطلحات موضوعية: | Animals, Gene Expression Profiling/methods, High-Throughput Nucleotide Sequencing/methods, Humans, Mice, RNA/genetics, RNA, Messenger/genetics, Reproducibility of Results, Sequence Analysis, RNA/methods, Single-Cell Analysis/methods, T-Lymphocytes, Cytotoxic/cytology, Cytotoxic/metabolism |
| الوصف: | Single-cell RNA sequencing in principle offers unique opportunities to improve the efficacy of contemporary T-cell based immunotherapy against cancer. The use of high-quality single-cell data will aid our incomplete understanding of molecular programs determining the differentiation and functional heterogeneity of cytotoxic T lymphocytes (CTLs), allowing for optimal therapeutic design. So far, a major obstacle to high depth single-cell analysis of CTLs is the minute amount of RNA available, leading to low capturing efficacy. Here, to overcome this, we tailor a droplet-based approach for high-throughput analysis (tDrop-seq) and a plate-based method for high-performance in-depth CTL analysis (tSCRB-seq). The latter gives, on average, a 15-fold higher number of captured transcripts per gene compared to droplet-based technologies. The improved dynamic range of gene detection gives tSCRB-seq an edge in resolution sensitive downstream applications such as graded high confidence gene expression measurements and cluster characterization. We demonstrate the power of tSCRB-seq by revealing the subpopulation-specific expression of co-inhibitory and co-stimulatory receptor targets of key importance for immunotherapy. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/33495472; info:eu-repo/semantics/altIdentifier/eissn/2041-1723; info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_C79F07ADC7C79; https://serval.unil.ch/notice/serval:BIB_C79F07ADC7C7; https://serval.unil.ch/resource/serval:BIB_C79F07ADC7C7.P001/REF.pdf |
| DOI: | 10.1038/s41467-020-20751-7 |
| الاتاحة: | https://serval.unil.ch/notice/serval:BIB_C79F07ADC7C7 https://doi.org/10.1038/s41467-020-20751-7 https://serval.unil.ch/resource/serval:BIB_C79F07ADC7C7.P001/REF.pdf http://nbn-resolving.org/urn/resolver.pl?urn=urn:nbn:ch:serval-BIB_C79F07ADC7C79 |
| Rights: | info:eu-repo/semantics/openAccess ; CC BY 4.0 ; https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.B4516A3C |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41467-020-20751-7 |
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