Academic Journal
Conjugated polymers mediate intracellular Ca2+ signals in circulating endothelial colony forming cells through the reactive oxygen species-dependent activation of Transient Receptor Potential Vanilloid 1 (TRPV1)
| العنوان: | Conjugated polymers mediate intracellular Ca2+ signals in circulating endothelial colony forming cells through the reactive oxygen species-dependent activation of Transient Receptor Potential Vanilloid 1 (TRPV1) |
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| المؤلفون: | Negri S, Faris P, Tullii G, Vismara M, Pellegata AFM, Lodola F, Guidetti G, Rosti V, Antognazza MR, Moccia F. |
| المساهمون: | Negri, S, Faris, P, Tullii, G, Vismara, M, Pellegata, Afm, Lodola, F, Guidetti, G, Rosti, V, Antognazza, Mr, Moccia, F. |
| سنة النشر: | 2022 |
| المجموعة: | RE.PUBLIC@POLIMI - Research Publications at Politecnico di Milano |
| مصطلحات موضوعية: | Conjugated polymers, Endothelial colony forming cells, Optical stimulation, Reactive oxygen species, Transient receptor potential vanilloid 1, Endoplasmic Reticulum, Endothelial Cells, TRPV Cation Channels, Calcium, Polymers |
| الوصف: | Endothelial colony forming cells (ECFCs) represent the most suitable cellular substrate to induce revascularization of ischemic tissues. Recently, optical excitation of the light-sensitive conjugated polymer, regioregular Poly (3-hexyl-thiophene), rr-P3HT, was found to stimulate ECFC proliferation and tube formation by activating the non-selective cation channel, Transient Receptor Potential Vanilloid 1 (TRPV1). Herein, we adopted a multidisciplinary approach, ranging from intracellular Ca2+ imaging to pharmacological manipulation and genetic suppression of TRPV1 expression, to investigate the effects of photoexcitation on intracellular Ca2+ concentration ([Ca2+](i)) in circulating ECFCs plated on rr-P3HT thin films. Polymer-mediated optical excitation induced a long-lasting increase in [Ca2+](i) that could display an oscillatory pattern at shorter light stimuli. Pharmacological and genetic manipulation revealed that the Ca2+ response to light was triggered by extracellular Ca2+ entry through TRPV1, whose activation required the production of reactive oxygen species at the interface between rr-P3HT and the cell membrane. Light-induced TRPV1-mediated Ca2+ entry was able to evoke intracellular Ca2+ release from the endoplasmic reticulum through inositol-1,4,5-trisphosphate receptors, followed by store-operated Ca2+ entry on the plasma membrane. These data show that TRPV1 may serve as a decoder at the interface between rr-P3HT thin films and ECFCs to translate optical excitation in pro-angiogenic Ca2+ signals. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/34896699; info:eu-repo/semantics/altIdentifier/wos/WOS:000737896500002; volume:101; firstpage:1; lastpage:5; numberofpages:5; journal:CELL CALCIUM; https://hdl.handle.net/11311/1221545; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85120869396 |
| DOI: | 10.1016/j.ceca.2021.102502 |
| الاتاحة: | https://hdl.handle.net/11311/1221545 https://doi.org/10.1016/j.ceca.2021.102502 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.B3287F |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.ceca.2021.102502 |
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