التفاصيل البيبلوغرافية
| العنوان: |
The Hirudo Medicinalis Microbiome Is a Source of New Antimicrobial Peptides |
| المؤلفون: |
Ekaterina Grafskaia, Elizaveta Pavlova, Vladislav V. Babenko, Ivan Latsis, Maja Malakhova, Victoria Lavrenova, Pavel Bashkirov, Dmitrii Belousov, Dmitry Klinov, Vassili Lazarev |
| المصدر: |
International Journal of Molecular Sciences; Volume 21; Issue 19; Pages: 7141 |
| بيانات النشر: |
Multidisciplinary Digital Publishing Institute |
| سنة النشر: |
2020 |
| المجموعة: |
MDPI Open Access Publishing |
| مصطلحات موضوعية: |
microbiome, metagenome, Hirudo medicinalis, antimicrobial peptides, bioinformatic analysis, secondary structure, circular dichroism, α–helix |
| جغرافية الموضوع: |
agris |
| الوصف: |
Antimicrobial peptides (AMPs) are considered a promising new class of anti-infectious agents. This study reports new antimicrobial peptides derived from the Hirudo medicinalis microbiome identified by a computational analysis method applied to the H. medicinalis metagenome. The identified AMPs possess a strong antimicrobial activity against Gram-positive and Gram-negative bacteria (MIC range: 5.3 to 22.4 μM), including Staphylococcus haemolyticus, an opportunistic coagulase–negative pathogen. The secondary structure analysis of peptides via CD spectroscopy showed that all the AMPs except pept_352 have mostly disordered structures that do not change under different conditions. For peptide pept_352, the α–helical content increases in the membrane environment. The examination of the mechanism of action of peptides suggests that peptide pept_352 exhibits a direct membranolytic activity. Furthermore, the cytotoxicity assay demonstrated that the nontoxic peptide pept_1545 is a promising candidate for drug development. Overall, the analysis method implemented in the study may serve as an effective tool for the identification of new AMPs. |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
Molecular Microbiology; https://dx.doi.org/10.3390/ijms21197141 |
| DOI: |
10.3390/ijms21197141 |
| الاتاحة: |
https://doi.org/10.3390/ijms21197141 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: |
edsbas.B319F315 |
| قاعدة البيانات: |
BASE |