Academic Journal
Mechanistic Elucidation of green seaweed compounds in orthodontic relapse management via RANKL/TNF-α-mediated ROS/Keap1/Nrf2 signaling: In silico and Ex Vivo studies
| العنوان: | Mechanistic Elucidation of green seaweed compounds in orthodontic relapse management via RANKL/TNF-α-mediated ROS/Keap1/Nrf2 signaling: In silico and Ex Vivo studies |
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| المؤلفون: | Ananto Ali Alhasyimi, Alexander Patera Nugraha, Aulia Ayub, Trianna Wahyu Utami, Timothy Sahala Gerardo, Nuril Farid Abshori, Mohammad Adib Khumaidi, Trina Ekawati Tallei, Nurpudji Astuti Taslim, Bonglee Kim, Raymond Rubianto Tjandrawinata, Apollinaire Tsopmo, Fahrul Nurkolis |
| المصدر: | Journal of Agriculture and Food Research, Vol 18, Iss , Pp 101396- (2024) |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2024 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Orthodontic relapse, Green seaweed, Antioxidants, RANKL, ROS/Keap1/Nrf2, Agriculture (General), S1-972, Nutrition. Foods and food supply, TX341-641 |
| الوصف: | Orthodontic relapse, the return to a pre-treatment position after orthodontic correction, is driven by the RANKL/TNF-α-mediated ROS/Keap1/Nrf2 signaling axis. This mechanism triggers aseptic inflammation and oxidative stress, influencing bone resorption and formation. Antioxidants can mitigate oxidative stress, potentially improving post-orthodontic outcomes. This study explores the efficacy of antioxidant compounds derived from green seaweed/algae in managing orthodontic relapse. Green seaweed/algae extracts were prepared via sonication, and bioactive compounds were identified using ultra-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS) analysis. Compounds underwent bioactivity prediction, toxicity assessment, and drug-likeness evaluation, revealing significant therapeutic potential. Network pharmacology and molecular docking identified key proteins associated with orthodontic relapse, including IL-1β, STAT3, ESR1, MAPK1, JAK2, and HMOX1. Molecular docking simulations indicated favorable binding energies for green seaweed compounds, particularly the alkaloids adenosine (ΔG −6.9 to −7.3 kcal/mol) and lycopodine (ΔG −6.3 to −8.5 kcal/mol), against targeted proteins, matching or outperforming standard drugs such as s-ibuprofen (ΔG −6.7 kcal/mol). In vitro assays confirmed the antioxidant activity of these compounds, with EC50 dose of 52.2–54.2 μg/mL for ABTS radical scavenging capacities. Protein expression analysis in tibial-femoral bone marrow cells further demonstrated the potential of green seaweed/algae compounds to suppress osteoclastogenesis by modulating the RANKL/TNF-α-mediated ROS/Keap1/Nrf2 pathway. This research highlights the promise of green seaweed-derived antioxidants in reducing oxidative stress and managing orthodontic relapse, providing a foundation for future therapeutic developments. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2666154324004332; https://doaj.org/toc/2666-1543; https://doaj.org/article/54157b70d41e4b228a41fe4b56f2c82e |
| DOI: | 10.1016/j.jafr.2024.101396 |
| الاتاحة: | https://doi.org/10.1016/j.jafr.2024.101396 https://doaj.org/article/54157b70d41e4b228a41fe4b56f2c82e |
| رقم الانضمام: | edsbas.B2FA2B50 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.jafr.2024.101396 |
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