Academic Journal
Exploring the cellular surface polysaccharide and root nodule symbiosis characteristics of the rpoN mutants of Bradyrhizobium sp. DOA9 using synchrotron-based Fourier transform infrared microspectroscopy in conjunction with X-ray absorption spectroscopy
| العنوان: | Exploring the cellular surface polysaccharide and root nodule symbiosis characteristics of the rpoN mutants of Bradyrhizobium sp. DOA9 using synchrotron-based Fourier transform infrared microspectroscopy in conjunction with X-ray absorption spectroscopy |
|---|---|
| المؤلفون: | Wongdee, Jenjira, Piromyou, Pongdet, Songwattana, Pongpan, Greetatorn, Teerana, Boonkerd, Nantakorn, Teaumroong, Neung, Giraud, Eric, Gully, Djamel, Nouwen, Nico, Kiatponglarp, Worawikunya, Tanthanuch, Waraporn, Tittabutr, Panlada |
| المساهمون: | Suranaree University of Technology (SUT), Plant Health Institute of Montpellier (UMR PHIM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM), Chulalongkorn University Bangkok, Synchrotron Light Research Institute (SLRI), National Science, Research and Innovation Fund (NSRF) (project code 160347), Program Management Unit for Human Resources & Institutional Development and Innovation (PMU-B) grant number B16F640113. |
| المصدر: | ISSN: 2165-0497 ; Microbiology Spectrum ; https://hal.inrae.fr/hal-04456141 ; Microbiology Spectrum, 2023, 11, pp.e01947-23. ⟨10.1128/spectrum.01947-23⟩. |
| بيانات النشر: | HAL CCSD American Society for Microbiology |
| سنة النشر: | 2023 |
| المجموعة: | Université de Montpellier: HAL |
| مصطلحات موضوعية: | [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy |
| الوصف: | International audience ; The functional significance of rpoN genes that encode two sigma factors in the Bradyrhizobium sp. strain DOA9 has been reported to affect colony formation, root nodulation characteristics, and symbiotic interactions with Aeschynomene americana. rpoN mutant strains are defective in cellular surface polysaccharide (CSP) production compared with the wild-type (WT) strain, and they accordingly exhibit smaller colonies and diminished symbiotic effectiveness. To gain deeper insights into the changes in CSP composition and the nodules of rpoN mutants, we employed synchrotron-based Fourier transform infrared (SR-FTIR) microspectroscopy and X-ray absorption spectroscopy. FTIR analysis of the CSP revealed the absence of specific components in the rpoN mutants, including lipids, carboxylic groups, polysaccharide-pyranose rings, and β-galactopyrano syl residues. Nodules formed by DOA9WT exhibited a uniform distribution of lipids, proteins, and carbohydrates; mutant strains, particularly DOA9∆rpoNp:ΩrpoNc, exhibited decreased distribution uniformity and a lower concentration of C=O groups. Further more, Fe K-edge X-ray absorption near-edge structure and extended X-ray absorption fine structure analyses revealed deficiencies in the nitrogenase enzyme in the nod ules of DOA9∆rpoNc and DOA9∆rpoNp:ΩrpoNc mutants; nodules from DOA9WT and DOA9∆rpoNp exhibited both leghemoglobin and the nitrogenase enzyme. IMPORTANCE This work provides valuable insights into how two rpoN genes affect the composition of cellular surface polysaccharides (CSPs) in Bradyrhizobium sp., which subsequently dictates root nodule chemical characteristics and nitrogenase production. We used advanced synchrotron methods, including synchrotron-based Fourier transform infrared (SR-FTIR) microspectroscopy and X-ray absorption spectroscopy (XAS), for the first time in this field to analyze CSP components and reveal the biochemical changes occurring within nodules. These cutting-edge techniques confer significant advantages by providing ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | WOS: 001064151500001 |
| DOI: | 10.1128/spectrum.01947-23 |
| الاتاحة: | https://hal.inrae.fr/hal-04456141 https://hal.inrae.fr/hal-04456141v1/document https://hal.inrae.fr/hal-04456141v1/file/Wongdee-MS-2023-CC-BY.pdf https://doi.org/10.1128/spectrum.01947-23 |
| Rights: | http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.B2D1B2B |
| قاعدة البيانات: | BASE |
| DOI: | 10.1128/spectrum.01947-23 |
|---|