التفاصيل البيبلوغرافية
| العنوان: |
Highly Efficient Transfection of Human Primary T Lymphocytes Using Droplet-Enabled Mechanoporation |
| المؤلفون: |
Byeongju Joo (10993496), Jeongsoo Hur (9503801), Gi-Beom Kim (10993499), Seung Gyu Yun (5737766), Aram J. Chung (1791292) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Medicine, Cell Biology, Molecular Biology, Pharmacology, Cancer, Space Science, Biological Sciences not elsewhere classified, Physical Sciences not elsewhere classified, droplet-based intracellular deliver., polymer nanocarriers, Droplet-Enabled Mechanoporation Who., intracellular delivery, recirculation flows, plasmid DNAs, genome modification, cell membrane, cell-based therapies, cargo consumption, disease treatment approach, droplets, T Lymphocytes, cell reprogramming methodologies, cell viability, mechanoporation microfluidic techniques, cell mechanoporation, macromolecule delivery, T cells, leverages droplet microfluidics, benchtop techniques |
| الوصف: |
Whole-cell-based therapy has been extensively used as an effective disease treatment approach, and it has rapidly changed the therapeutic paradigm. To fully accommodate this shift, advances in genome modification and cell reprogramming methodologies are critical. Traditionally, molecular tools such as viral and polymer nanocarriers and electroporation have been the norm for internalizing external biomolecules into cells for cellular engineering. However, these approaches are not fully satisfactory considering their cytotoxicity, high cost, low scalability, and/or inconsistent and ineffective delivery and transfection. To address these challenges, we present an approach that leverages droplet microfluidics with cell mechanoporation, bringing intracellular delivery to the next level. In our approach, cells and external cargos such as mRNAs and plasmid DNAs are coencapsulated into droplets, and as they pass through a series of narrow constrictions, the cell membrane is mechanically permeabilized where the cargos in the vicinity are internalized via convective solution exchange enhanced by recirculation flows developed in the droplets. Using this principle, we demonstrated a high level of functional macromolecule delivery into various immune cells, including human primary T cells. By utilizing droplets, the cargo consumption was drastically reduced, and near-zero clogging was realized. Furthermore, high scalability without sacrificing cell viability and superior delivery over state-of-the-art methods and benchtop techniques were demonstrated. Notably, the droplet-based intracellular delivery strategy presented here can be further applied to other mechanoporation microfluidic techniques, highlighting its potential for cellular engineering and cell-based therapies. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/journal_contribution/Highly_Efficient_Transfection_of_Human_Primary_T_Lymphocytes_Using_Droplet-Enabled_Mechanoporation/14807303 |
| DOI: |
10.1021/acsnano.0c10473.s001 |
| الاتاحة: |
https://doi.org/10.1021/acsnano.0c10473.s001 |
| Rights: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.B294F362 |
| قاعدة البيانات: |
BASE |