التفاصيل البيبلوغرافية
| العنوان: |
Highly Stable Covalent Organic Framework Nanosheets as a New Generation of Electrochemiluminescence Emitters for Ultrasensitive MicroRNA Detection |
| المؤلفون: |
Jin-Ling Zhang (1958035), Yang Yang (45629), Wen-Bin Liang (3045378), Li-Ying Yao (2415952), Ruo Yuan (1405666), Dong-Rong Xiao (2041750) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Medicine, Genetics, Molecular Biology, Evolutionary Biology, Ecology, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Information Systems not elsewhere classified, bulk Py-sp 2 c-COF, luminophore, imine-linked pyrene COF, ECL biosensor, Stable Covalent Organic Framework N., Bu 4 NPF 6, aggregation-caused quenching effect, aggregation-induced emissive luminogens, sp 2 carbon CONs, Ultrasensitive MicroRNA Detection, pyrene-based sp 2 carbon-conjugated., ECL emitter, ECL intensity, Py-sp 2 c-CON, microRNA -21 detection |
| الوصف: |
A pyrene-based sp 2 carbon-conjugated covalent organic framework (COF) nanosheet (Py-sp 2 c-CON) with strong and stable electrochemiluminescence (ECL) emission was constructed by CC polycondensation of tetrakis(4-formylphenyl)pyrene (TFPPy) and 2,2′-(1,4-phenylene)diacetonitrile, which was employed as a highly efficient ECL emitter to fabricate an ECL biosensor for the first time. The Py-sp 2 c-CON exhibited higher ECL intensity and efficiency than those of TFPPy, bulk Py-sp 2 c-COF, and imine-linked pyrene COF, not only because the pyrene luminophores and aggregation-induced emissive luminogens (cyano-substituted phenylenevinylene) were topologically linked into Py-sp 2 c-CON, which greatly increased the immobilization amount of luminophores and decreased the aggregation-caused quenching effect and nonradiative transition but also because the porous ultrathin structure of Py-sp 2 c-CON effectively shortened transport distances of an electron, ion, and co-reactant (S 2 O 8 2– ), which made more ECL luminophores be activated and thus efficiently increased the utilization ratio of luminophores. More interestingly, when Bu 4 NPF 6 was introduced into the Py-sp 2 c-CON/S 2 O 8 2– system as a co-reaction accelerator, the ECL signal of Py-sp 2 c-CON was further amplified. As expected, the average ECL intensity of the Py-sp 2 c-CON/S 2 O 8 2– /Bu 4 NPF 6 system was about 2.03, 5.76, 24.31, and 190.33-fold higher than those of Py-sp 2 c-CON/S 2 O 8 2– , Py-sp 2 c-COF/S 2 O 8 2– , TFPPy/S 2 O 8 2 , – and imine-linked pyrene COF/S 2 O 8 2– systems. Considering these advantages, the Py-sp 2 c-CON/S 2 O 8 2– /Bu 4 NPF 6 system was employed to prepare an ECL biosensor for microRNA-21 detection, which exhibited a broad linear response (100 aM to 1 nM) and a low detection limit (46 aM). Overall, this work demonstrated that sp 2 carbon CONs can be directly used as a high-performance ECL emitter, thus expanding the application scope of COFs and opening a new horizon to develop new types of ECL emitters. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/journal_contribution/Highly_Stable_Covalent_Organic_Framework_Nanosheets_as_a_New_Generation_of_Electrochemiluminescence_Emitters_for_Ultrasensitive_MicroRNA_Detection/13697411 |
| DOI: |
10.1021/acs.analchem.0c04931.s001 |
| الاتاحة: |
https://doi.org/10.1021/acs.analchem.0c04931.s001 |
| Rights: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.B2738805 |
| قاعدة البيانات: |
BASE |