التفاصيل البيبلوغرافية
| العنوان: |
Table1_One-pot encapsulation of lactate dehydrogenase and Fe3O4 nanoparticles into a metal–organic framework: A novel magnetic recyclable biocatalyst for the synthesis of D-phenyllactic acid.DOCX |
| المؤلفون: |
Xiaolong Sun (1726846), Jiahuan Hu (4906603), Yifeng Wang (117830), Xi Luo (361139), He Huang (50693), Yongqian Fu (1835221) |
| سنة النشر: |
2023 |
| مصطلحات موضوعية: |
Biotechnology, Biological Engineering, Genetic Engineering, Biomarkers, Biomaterials, Biomechanical Engineering, Biomedical Engineering not elsewhere classified, Synthetic Biology, Agricultural Marine Biotechnology, Bioremediation, Bioprocessing, Bioproduction and Bioproducts, Industrial Biotechnology Diagnostics (incl. Biosensors), Industrial Microbiology (incl. Biofeedstocks), Industrial Molecular Engineering of Nucleic Acids and Proteins, Industrial Biotechnology not elsewhere classified, Medical Biotechnology Diagnostics (incl. Biosensors), Medical Molecular Engineering of Nucleic Acids and Proteins, Regenerative Medicine (incl. Stem Cells and Tissue Engineering), Medical Biotechnology not elsewhere classified, biocatalyst, d-phenyllactic acid, lactate dehydrogenase, LDH/MNPs@MAF-7, magnetic nanoparticles |
| الوصف: |
The main challenges in bio-catalysis of d-phenyllactic acid (D-PLA) are poor tolerance of lactate dehydrogenase (LDH) to harsh environmental conditions and inability to recycle the catalyst. A novel magnetic framework composite was prepared as solid support for the immobilization of enzymes via one-pot encapsulation in this study. LDH/MNPs@MAF-7 was synthesized by the one-pot encapsulation of both LDH and magnetic nanoparticles (MNPs) in MAF-7. The LDH/MNPs@MAF-7 showed stable biological activity for the efficient biosynthesis of D-PLA. The structure and morphology of LDH/MNPs@MAF-7 were systematically characterized by SEM, FT-IR, XRD, VSM, XPS, TGA and N 2 sorption. These indicated that LDH/MNPs@MAF-7 was successfully synthesized, exhibiting enhanced resistance to acid and alkali, temperature and organic solvents. Furthermore, the bio-catalyst could be separated easily using a magnet, and the reusability was once considerably expanded with 80% of enzyme activity last after eight rounds of recycling. Therefore, LDH/MNPs@MAF-7 could be used as a potential biocatalyst for the biosynthesis of D-PLA due to its good stability and recovery properties. |
| نوع الوثيقة: |
dataset |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/dataset/Table1_One-pot_encapsulation_of_lactate_dehydrogenase_and_Fe3O4_nanoparticles_into_a_metal_organic_framework_A_novel_magnetic_recyclable_biocatalyst_for_the_synthesis_of_D-phenyllactic_acid_DOCX/21838026 |
| DOI: |
10.3389/fbioe.2022.1124450.s001 |
| الاتاحة: |
https://doi.org/10.3389/fbioe.2022.1124450.s001 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.B2613E54 |
| قاعدة البيانات: |
BASE |