Academic Journal
A comparison of nucleoside ({beta}-S) triphosphates and nucleoside ({gamma}-S) triphosphates as suitable substrates for measuring transcription initiation in preparations of cell nuclei
| العنوان: | A comparison of nucleoside ({beta}-S) triphosphates and nucleoside ({gamma}-S) triphosphates as suitable substrates for measuring transcription initiation in preparations of cell nuclei |
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| المؤلفون: | Washington, Lynette D., Stallcup, Michael R. |
| بيانات النشر: | Oxford University Press |
| سنة النشر: | 1982 |
| المجموعة: | HighWire Press (Stanford University) |
| مصطلحات موضوعية: | MOLECULAR BIOLOGY |
| الوصف: | RNA chains initiated with nucleoside (β-S)triphosphates and (γ-S)triphosphates retain the thiol groups and can be separated from thiol-free RNA by chromatography on mercury-Sepharose. Thiol-containing mouse mammary tumor virus (MMTV) RNA synthesized by preparations of nuclei from virus-infected cells was quantitated by nucleic acid filter hybridization. With ATPβS and GTPβS, region-specific initiation of MMTV RNA chains was detected in the cell free system. However, with ATPγS and GTPγS, region-specific initiation was not clearly demonstrable. The nuclear preparations can also transfer thiol groups, presumably in the form of thiophosphate, from ATPγS or GTPγS onto preexisting RNA molecules; little or no thiol-transfer occurs with the two (β-S)-analogues. The thiophosphate transfer activity apparently interferes with the measurement of RNA chain initiation with ATPγS and GTPγS. |
| نوع الوثيقة: | text |
| وصف الملف: | text/html |
| اللغة: | English |
| Relation: | http://nar.oxfordjournals.org/cgi/content/short/10/24/8311; http://dx.doi.org/10.1093/nar/10.24.8311 |
| DOI: | 10.1093/nar/10.24.8311 |
| الاتاحة: | http://nar.oxfordjournals.org/cgi/content/short/10/24/8311 https://doi.org/10.1093/nar/10.24.8311 |
| Rights: | Copyright (C) 1982, Oxford University Press |
| رقم الانضمام: | edsbas.B222558B |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/nar/10.24.8311 |
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