Academic Journal
Analysis of the Molecular Dialogue Between Gray Mold (Botrytis cinerea) and Grapevine (Vitis vinifera) Reveals a Clear Shift in Defense Mechanisms During Berry Ripening.
| العنوان: | Analysis of the Molecular Dialogue Between Gray Mold (Botrytis cinerea) and Grapevine (Vitis vinifera) Reveals a Clear Shift in Defense Mechanisms During Berry Ripening. |
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| المؤلفون: | Jean-Marc Pradier, Mathias Choquer, Agnès Cimerman, Sophie Trouvelot, Guillaume Morgant, Lucie Trdá, Marc Fermaud, Christophe Bruel, Adeline Simon, Muriel Viaud, Nathalie Poussereau, Alberto Ferrarini, Bérengère Dalmais, Sylvain Baulande, Victor Flors, Mathilde Duthieuw, Jani Kelloniemi, Jean Roudet, Christine Rascle, Marie-Claire Héloir, Elise Loisel, Patrick Frettinger, Benoît Poinssot, Pascal Le Pêcheur, Linhdavanh Couvelard |
| سنة النشر: | 2015 |
| المجموعة: | Istituto Nazionale di Fisica Nucleare (INFN): Open Access Repository |
| مصطلحات موضوعية: | FORTHEM Alliance, Knowmad Institut, Agronomy and Crop Science, General Medicine, Physiology |
| الوصف: | Mature grapevine berries at the harvesting stage (MB) are very susceptible to the gray mold fungus Botrytis cinerea, while veraison berries (VB) are not. We conducted simultaneous microscopic and transcriptomic analyses of the pathogen and the host to investigate the infection process developed by B. cinerea on MB versus VB, and the plant defense mechanisms deployed to stop the fungus spreading. On the pathogen side, our genome-wide transcriptomic data revealed that B. cinerea genes upregulated during infection of MB are enriched in functional categories related to necrotrophy, such as degradation of the plant cell wall, proteolysis, membrane transport, reactive oxygen species (ROS) generation, and detoxification. Quantitative-polymerase chain reaction on a set of representative genes related to virulence and microscopic observations further demonstrated that the infection is also initiated on VB but is stopped at the penetration stage. On the plant side, genome-wide transcriptomic analysis and metabolic data revealed a defense pathway switch during berry ripening. In response to B. cinerea inoculation, VB activated a burst of ROS, the salicylate-dependent defense pathway, the synthesis of the resveratrol phytoalexin, and cell-wall strengthening. On the contrary, in infected MB, the jasmonate-dependent pathway was activated, which did not stop the fungal necrotrophic process. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | url:https://www.openaccessrepository.it/communities/itmirror; https://www.openaccessrepository.it/record/101259 |
| DOI: | 10.1094/mpmi-02-15-0039-r |
| الاتاحة: | https://www.openaccessrepository.it/record/101259 https://doi.org/10.1094/mpmi-02-15-0039-r |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.B21C87A |
| قاعدة البيانات: | BASE |
| DOI: | 10.1094/mpmi-02-15-0039-r |
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