التفاصيل البيبلوغرافية
| العنوان: |
Additional file 2 of Glacier ice archives nearly 15,000-year-old microbes and phages |
| المؤلفون: |
Zhi-Ping Zhong (11155886), Funing Tian (11155889), Simon Roux (151891), M. Consuelo Gazitúa (10066679), Natalie E. Solonenko (5280548), Yueh-Fen Li (11155892), Mary E. Davis (11155895), James L. Van Etten (8858468), Ellen Mosley-Thompson (5280533), Virginia I. Rich (5280542), Matthew B. Sullivan (7419248), Lonnie G. Thompson (5280545) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Microbiology, Genetics, Guliya ice cap, Mountain glacier ice, Surface decontamination, Ice microbes, Ice viruses, Methylobacterium, Sphingomonas, Janthinobacterium |
| الوصف: |
Additional file2: Figure S1 Ice core sampling and preparation in the laboratory. (a) The cold work room (−5°C) with band saw, BioGard laminar flow hood and wash systems. (b) the outer layer of the ice section being removed by the band saw. (c) The ice section being washed with 95% ethanol and (d) with water. (e) The “clean” inner ice is preserved in the autoclaved beakers or bottles. Figure S2 Microbial communities at genus level (a) and overlapped OTUs (b) of removed and inner ice samples collected during decontamination procedures. The most abundant genera (n = 30) and OTUs (n = 33) are illustrated. Cut, Wash and Inner represent ice samples collected from band saw scrapping, water washing and the inner ice, respectively. Figure S3 Rarefaction curves of two glacier-ice viromes by vOTU numbers. Rarefaction curves were constructed by the change of vOTUs (≥10 kb) number along sequencing depth (i.e., read number) obtained by subsampling quality-controlled reads. Figure S4 The unrooted neighbor-joining phylogenetic tree of Mu N genes from eight Methylobacterium viruses. The tree was constructed using the predicted amino acid sequences of the N genes from two glacier ice viruses (i.e., D25_14_65719 and D49_170_39214; in bold font) and six prophages identified from bacterial genomes. Each viral contig contains two copies of N genes. Viruses belonged to the same VC (i.e., VC0_0 or VC8_0) are indicated in the same color. Bootstrap values (expressed as percentages of 1,000 replications) are shown at the branch points. The scale bar indicates a distance of 0.2. Figure S5 Characterization of virus-encoded auxiliary metabolic genes (AMGs). (a) Genome map of glacier-ice virus D25_22_20338 encoding AMGs (motility genes motA and motB). CheckV was used to assess host-virus boundaries and remove potential host fractions on the viral contig (See Materials and Methods). Genes were marked by four colors to illustrate AMGs (red), phage genes (orange), potential cellular genes (green), and unaffiliated genes (grey). AMGs were ... |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/journal_contribution/Additional_file_2_of_Glacier_ice_archives_nearly_15_000-year-old_microbes_and_phages/15020420 |
| DOI: |
10.6084/m9.figshare.15020420.v1 |
| الاتاحة: |
https://doi.org/10.6084/m9.figshare.15020420.v1 |
| Rights: |
CC BY + CC0 |
| رقم الانضمام: |
edsbas.B1D734DF |
| قاعدة البيانات: |
BASE |