التفاصيل البيبلوغرافية
| العنوان: |
Depression Mediated By Inflammatory Responses To Chronic Stress |
| المؤلفون: |
Kokkosis, Alexandros, Valais, Kimonas, Mullahy, Matthew, Tsirka, Stella E. |
| المصدر: |
The FASEB Journal ; volume 34, issue S1, page 1-1 ; ISSN 0892-6638 1530-6860 |
| بيانات النشر: |
Wiley |
| سنة النشر: |
2020 |
| المجموعة: |
Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: |
Background Major depressive disorder (MDD) is a chronic debilitating illness affecting yearly 350 million people worldwide. Although the mechanisms underlying depression are still not defined, it has been suggested that inflammation acts as depression mediator promoting glial and neuronal dysfunction. Microglia, as the resident innate immune cells of the brain, can be activated by both immune (i.e. infection) and nonimmune challenges (i.e. psychosocial stress), and contribute to the regulation of neurological and neuropsychiatric disorders. The aim of the present study is to characterize the inflammatory responses during chronic social stress and determine their effects on neuronal homeostasis and depression in a rodent depression model. Methods The Repeated Social Defeat Stress (RSDS) paradigm (10 days) was utilized to study the post RSDS stages [(10+5 days (D15) and 10+15 days (D25)] in 2–4 months old male C57BL/6J, CX3CR1 ‐GFP + mice. At the beginning of the RSDS paradigm the mice were fed with chow containing the Csf1R inhibitors PLX5622 [passes blood brain barrier (BBB) and specifically targets microglia] or PLX73086 (does not cross BBB and targets peripheral monocytes) or control chow. In addition, all groups were administered BrdU (5‐bromo‐2’‐deoxyuridine) ad libitum to monitor cell proliferation. Behavioral tasks for social interaction, anxiety, anhedonia and despair behavioral (BH1 & BH2) were performed to categorize the defeated mice to susceptible (S; depressive‐like) and resilient (R; non‐depressive) to stress groups. The study focuses on the MDD‐affected Prefrontal (mPFC), Ventral or Lateral Orbital (VO/LO) Cortex areas. Cell quantification and data analysis were partially blinded and performed by 3 investigators. Results Inflammation was observed in all areas at D15, depicted by the presence of activated Iba1 + cells [reactive morphology (CD68 + ) and inflammatory markers (TSPO, CD206, CD86, iNOS, Arg‐1)] in the S groups. Given the increased microglial numbers, microglial proliferation capacity ... |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1096/fasebj.2020.34.s1.02479 |
| الاتاحة: |
http://dx.doi.org/10.1096/fasebj.2020.34.s1.02479 |
| Rights: |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: |
edsbas.B1D4EBEE |
| قاعدة البيانات: |
BASE |