Academic Journal
Virulent pseudorabies virus infection induces a specific and lethal systemic inflammatory response in mice
| العنوان: | Virulent pseudorabies virus infection induces a specific and lethal systemic inflammatory response in mice |
|---|---|
| المؤلفون: | Laval, Kathlyn, Vernejoul, J. B., Van Cleemput, Jolien, Koyuncu, O. O., Enquist, L. W. |
| المصدر: | JOURNAL OF VIROLOGY ; ISSN: 0022-538X ; ISSN: 1098-5514 |
| سنة النشر: | 2018 |
| المجموعة: | Ghent University Academic Bibliography |
| مصطلحات موضوعية: | Biology and Life Sciences, C-REACTIVE PROTEIN, AUJESZKYS-DISEASE, VACCINE STRAIN, NEUTROPHIL, MIGRATION, HERPES-SIMPLEX, INFLUENZA, PATHOGENESIS, INJURY, CELL, EMERGENCE, pseudorabies virus, cytokines, nervous system, mice, immunopathogenesis, inflammation |
| الوصف: | Pseudorabies virus (PRV) is an alphaherpesvirus that infects the peripheral nervous system (PNS). The natural host of PRV is the swine, but it can infect most mammals, including cattle, rodents, and dogs. In these nonnatural hosts, PRV always causes a severe acute and lethal neuropathy called the "mad itch," which is uncommon in swine. Thus far, the pathophysiological and immunological processes leading to the development of the neuropathic itch and the death of the animal are unclear. Using a footpad inoculation model, we established that mice inoculated with PRV-Becker (virulent strain) develop a severe pruritus in the foot and become moribund at 82 h postinoculation (hpi). We found necrosis and inflammation with a massive neutrophil infiltration only in the footpad and dorsal root ganglia (DRGs) by hematoxylin and eosin staining. PRV load was detected in the foot, PNS, and central nervous system tissues by quantitative reverse transcription-PCR. Infected mice had elevated plasma levels of proinflammatory cytokines (interleukin-6 [IL-6] and granulocyte colony-stimulating factor [G-CSF]) and chemokines (Gro-1 and monocyte chemoattractant protein 1). Significant IL-6 and G-CSF levels were detected in several tissues at 82 hpi. High plasma levels of C-reactive protein confirmed the acute inflammatory response to PRV-Becker infection. Moreover, mice inoculated with PRV-Bartha (attenuated, live vaccine strain) did not develop pruritus at 82 hpi. PRV-Bartha also replicated in the PNS, and the infection spread further in the brain than PRV-Becker. PRV-Bartha infection did not induce the specific and lethal systemic inflammatory response seen with PRV-Becker. Overall, we demonstrated the importance of inflammation in the clinical outcome of PRV infection in mice and provide new insights into the process of PRV-induced neuroinflammation. IMPORTANCE Pseudorabies virus (PRV) is an alphaherpesvirus related to human pathogens such as herpes simplex virus 1 and varicella-zoster virus (VZV). The natural host of PRV is the ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | https://biblio.ugent.be/publication/8712235; http://hdl.handle.net/1854/LU-8712235; http://dx.doi.org/10.1128/JVI.01614-18; https://biblio.ugent.be/publication/8712235/file/8712238 |
| DOI: | 10.1128/JVI.01614-18 |
| الاتاحة: | https://biblio.ugent.be/publication/8712235 http://hdl.handle.net/1854/LU-8712235 https://doi.org/10.1128/JVI.01614-18 https://biblio.ugent.be/publication/8712235/file/8712238 |
| Rights: | No license (in copyright) ; info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: | edsbas.B126BCE6 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1128/JVI.01614-18 |
|---|