Academic Journal
A fast, sensitive and cost-effective method for nucleic acid detection using non-radioactive probes
| العنوان: | A fast, sensitive and cost-effective method for nucleic acid detection using non-radioactive probes |
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| المؤلفون: | Viterbo, David, Marchal, Astrid, Mosbach, Valentine, Poggi, Lucie, Vaysse-Zinkhöfer, Wilhelm, Richard, Guy-Franck |
| المساهمون: | Dynamique du Génome - Dynamics of the genome, Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS), Collège Doctoral, Sorbonne Université (SU), SANOFI Recherche, V.M. was supported by Fondation Guy Nicolas and Fondation Hardy. L.P. is the recipient of a PhD student CIFRE fellowship from SANOFI. W.V.-Z. is the recipient of a PhD student fellowship from the Ligue Nationale contre le Cancer. |
| المصدر: | EISSN: 2396-8923 ; Biology Methods and Protocols ; https://pasteur.hal.science/pasteur-02864565 ; Biology Methods and Protocols, 2018, ⟨10.1093/biomethods/bpy006⟩ |
| بيانات النشر: | HAL CCSD Oxford University Press |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | nucleic acid hybridization, nucleic acid quantification, digoxigenin-labeled probe, [SDV]Life Sciences [q-bio] |
| الوصف: | International audience ; Nucleic acid detection and quantification using a labeled DNA probe is a very common molecular biology procedure. Here, we describe a new method, based on commonly used laboratory solutions, for nucleic acid hybridization and detection with digoxigenin-labeled DNA probes. The protocol described is faster, more sensitive and much cheaper than a standard protocol using commercial solutions. Comparison with a classical radioactive detection method shows that the latter exhibits less background and shows a greater linear response. Hence, the proposed protocol may be routinely performed for qualitative detection of nucleic acid, but when precise signal quantitation needs to be obtained, radioactive probe hybridization associated to phosphorimaging technology is more reliable. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/32161800; pasteur-02864565; https://pasteur.hal.science/pasteur-02864565; https://pasteur.hal.science/pasteur-02864565/document; https://pasteur.hal.science/pasteur-02864565/file/2018-Viterbo%20%28DIG%20probes%29.pdf; PUBMED: 32161800; PUBMEDCENTRAL: PMC6994052 |
| DOI: | 10.1093/biomethods/bpy006 |
| الاتاحة: | https://pasteur.hal.science/pasteur-02864565 https://pasteur.hal.science/pasteur-02864565/document https://pasteur.hal.science/pasteur-02864565/file/2018-Viterbo%20%28DIG%20probes%29.pdf https://doi.org/10.1093/biomethods/bpy006 |
| Rights: | http://creativecommons.org/licenses/by-nc/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.B1159546 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/biomethods/bpy006 |
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