Academic Journal
In situ hybridisation of albumin mRNA in normal liver and hepatocellular carcinoma with a digoxigenin labelled oligonucleotide probe.
| العنوان: | In situ hybridisation of albumin mRNA in normal liver and hepatocellular carcinoma with a digoxigenin labelled oligonucleotide probe. |
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| المؤلفون: | Murray, G I, Paterson, P J, Ewen, S W, Melvin, W T |
| المصدر: | Journal of Clinical Pathology ; volume 45, issue 1, page 21-24 ; ISSN 0021-9746 1472-4146 |
| بيانات النشر: | BMJ |
| سنة النشر: | 1992 |
| الوصف: | AIMS: To study the localisation and distribution of albumin mRNA in normal liver and hepatocellular carcinoma by in situ hybridisation with an oligonucleotide probe. METHODS: A 51 base oligonucleotide was synthesised from a sequence at the 5' end of the human albumin gene and the probe was labelled at its 3' end with digoxigenin 11-dUTP. Formalin fixed, wax embedded sections of liver biopsy specimens were used to study the localisation and distribution of albumin mRNA. After in situ hybridisation the bound probe was visualised using a digoxigenin antibody conjugated with alkaline phosphatase. RESULTS: In normal liver albumin mRNA was detected in hepatocytes and no positive signal was observed in biliary epithelium, vascular endothelium, or Kupffer cells. In 75% (9/12) of the hepatocellular carcinomas studied a positive hybridisation signal was observed in tumour cells. CONCLUSIONS: Albumin mRNA can be detected in sections of formalin fixed, wax embedded liver, a digoxigenin labelled probe is ideally suited for in situ hybridisation of liver because there is no background from the detection system. The identification of albumin mRNA may be a useful marker of hepatocellular carcinoma, and the demonstration of albumin mRNA by in situ hybridisation overcomes the potential background problem associated with albumin immunohistochemistry. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1136/jcp.45.1.21 |
| الاتاحة: | http://dx.doi.org/10.1136/jcp.45.1.21 https://syndication.highwire.org/content/doi/10.1136/jcp.45.1.21 |
| رقم الانضمام: | edsbas.B092B247 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1136/jcp.45.1.21 |
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