Academic Journal
Cell-to-cell spread of wild-type herpes simplex virus type 1, but not of syncytial strains, is mediated by the immunoglobulin-like receptors that mediate virion entry, Nectin1 (PRR1/HveC/HIgR) and Nectin2 (PRR2/HveB)
| العنوان: | Cell-to-cell spread of wild-type herpes simplex virus type 1, but not of syncytial strains, is mediated by the immunoglobulin-like receptors that mediate virion entry, Nectin1 (PRR1/HveC/HIgR) and Nectin2 (PRR2/HveB) |
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| المؤلفون: | Cocchi F., Menotti L., Dubreuil P., Lopez M., Campadelli-Fiume G. |
| المساهمون: | Cocchi F., Menotti L., Dubreuil P., Lopez M., Campadelli-Fiume G. |
| سنة النشر: | 2000 |
| المجموعة: | IRIS Università degli Studi di Bologna (CRIS - Current Research Information System) |
| مصطلحات موضوعية: | Antibodies, Monoclonal/immunology Cell Adhesion Cell Adhesion Molecules/chemistry/immunology/*metabolism Cell Fusion Cell Line Giant Cells/physiology Herpesvirus 1, Human/genetics/pathogenicity/*physiology Humans Mutation Nectins Receptors, Virus/*metabolism Viral Envelope Proteins/genetics/metabolism Viral Plaque Assay Virion/physiology |
| الوصف: | The immunoglobulin-like receptors that mediate entry of herpes simplex virus type 1 (HSV-1) into human cells were found to mediate the direct cell- to-cell spread of wild-type virus. The receptors here designated Nectin1α and -δ and Nectin2α were originally designated HIgR, PRR1/HveC, and PRR2α/HveB, respectively. We report the following. (i) Wild-type HSV-1 spreads from cell to cell in J cells expressing nectin1α or nectin1δ but not in parental J cells that are devoid of entry receptors. A monoclonal antibody to nectin1, which blocks entry, also blocked cell-to-cell spread in nectin1-expressing J cells. Moreover, wild-type virus did not spread from a receptor-positive to a receptor-negative cell. (ii) The antibody to nectin1 blocked transmission of wild-type virus in a number of human cell lines, with varying efficiencies, suggesting that nectin1 is the principal mediator of wild-type virus spread in a variety of human cell lines. (iii) Nectin1 did not mediate cell fusion induced by the syncytial strains HSV-1(MP) and HFEM- syn. (iv) Nectin2α could serve as a receptor for spread of a mutant virus carrying the L25P substitution in glycoprotein D, but not of wild-type virus, in agreement with its ability to mediate entry of the mutant but not of wild- type virus. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | ELETTRONICO |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/10729168; info:eu-repo/semantics/altIdentifier/wos/WOS:000086048000053; volume:74; issue:8; firstpage:3909; lastpage:3917; numberofpages:9; journal:JOURNAL OF VIROLOGY; http://hdl.handle.net/11585/873897; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0034109367 |
| DOI: | 10.1128/JVI.74.8.3909-3917.2000 |
| الاتاحة: | http://hdl.handle.net/11585/873897 https://doi.org/10.1128/JVI.74.8.3909-3917.2000 |
| رقم الانضمام: | edsbas.B074226D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1128/JVI.74.8.3909-3917.2000 |
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