Academic Journal
Successful chilling of red-legged partridge (Alectoris rufa) sperm for use in artificial insemination
| العنوان: | Successful chilling of red-legged partridge (Alectoris rufa) sperm for use in artificial insemination |
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| المؤلفون: | Santiago Moreno, Julián, Castaño García, Cristina, Toledano Díaz, Adolfo, Esteso Diez, Milagros Cristina, López Sebastián, Antonio, Villaverde-Morcillo, S., García Dávila, Sara, Gil, M. G., Blesbois, E. |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2017 |
| المجموعة: | Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council) |
| مصطلحات موضوعية: | Artificial insemination, Semen preservation, Semen diluent, Red-legged partridge, Fertility |
| الوصف: | The fertilizing capacity of pure, fresh avian semen may disappear in just half an hour, hindering its successful use in artificial insemination (AI) projects. Longer storage requires the use of infra-physiological temperatures and of semen diluents that help preserve the spermatozoa but that do not interfere with their fertilizing capacity. This study examines the effect on sperm quality of storing red-legged partridge sperm for 3 h at 5°C with 2 different semen extenders: 1) a medium referred to as L&R-84, composed of sodium glutamate, glucose, magnesium acetate, potassium acetate, and polyvinylpyrrolidone, and 2) Lake 7.1 medium, composed of sodium glutamate, glucose, magnesium acetate, potassium citrate, and N,N-Bis(2-hydroxyethyl)taurine (BES). Extending with L&R-84 returned better curvilinear velocity (P < 0.01), straight-line velocity (P < 0.01), average path velocity (P < 0.01), linearity (P < 0.05), straightness (P < 0.05), and wobble (P < 0.05) values, while extending with the Lake 7.1 medium was associated with higher percentages (P < 0.001) of motile sperm. The fertility rate was higher (P < 0.05) when birds were inseminated with L&R-84-extended sperm than with Lake 7.1-extended sperm. The mean number of penetrations of perivitelline layer samples (taken from above the germinal disc) was also higher for the L&R-84-extended sperm (P < 0.05). These results show L&R-84 can be recommended as an extender for red-legged partridge semen to be stored for at least 3 h at 5°C. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0032-5791 1525-3171 |
| Relation: | Poultry Science 96: 4068-4074 (2017); http://hdl.handle.net/20.500.12792/4168; http://hdl.handle.net/10261/291303 |
| DOI: | 10.3382/ps/pex176 |
| الاتاحة: | https://hdl.handle.net/20.500.12792/4168 http://hdl.handle.net/10261/291303 https://doi.org/10.3382/ps/pex176 |
| Rights: | none |
| رقم الانضمام: | edsbas.B053F722 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 00325791 15253171 |
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| DOI: | 10.3382/ps/pex176 |