Academic Journal
Cell diversity and plasticity during atrioventricular heart valve EMTs
| العنوان: | Cell diversity and plasticity during atrioventricular heart valve EMTs |
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| المؤلفون: | Lotto, Jeremy, Cullum, Rebecca, Drissler, Sibyl, Arostegui, Martin, Garside, Victoria C., Fuglerud, Bettina M., Clement-Ranney, Makenna, Thakur, Avinash, Underhill, T. Michael, Hoodless, Pamela A. |
| المساهمون: | Gouvernement du Canada | Canadian Institutes of Health Research, Canadian Foundation for Innovation British Columbia Knowledge Development Fund |
| المصدر: | Nature Communications ; volume 14, issue 1 ; ISSN 2041-1723 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2023 |
| الوصف: | Epithelial-to-mesenchymal transitions (EMTs) of both endocardium and epicardium guide atrioventricular heart valve formation, but the cellular complexity and small scale of this tissue have restricted analyses. To circumvent these issues, we analyzed over 50,000 murine single-cell transcriptomes from embryonic day (E)7.75 hearts to E12.5 atrioventricular canals. We delineate mesenchymal and endocardial bifurcation during endocardial EMT, identify a distinct, transdifferentiating epicardial population during epicardial EMT, and reveal the activation of epithelial-mesenchymal plasticity during both processes. In Sox9- deficient valves, we observe increased epithelial-mesenchymal plasticity, indicating a role for SOX9 in promoting endothelial and mesenchymal cell fate decisions. Lastly, we deconvolve cell interactions guiding the initiation and progression of cardiac valve EMTs. Overall, these data reveal mechanisms of emergence of mesenchyme from endocardium or epicardium at single-cell resolution and will serve as an atlas of EMT initiation and progression with broad implications in regenerative medicine and cancer biology. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1038/s41467-023-41279-6 |
| الاتاحة: | http://dx.doi.org/10.1038/s41467-023-41279-6 https://www.nature.com/articles/s41467-023-41279-6.pdf https://www.nature.com/articles/s41467-023-41279-6 |
| Rights: | https://creativecommons.org/licenses/by/4.0 ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: | edsbas.B03A6004 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41467-023-41279-6 |
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