Academic Journal
Quantitative and qualitative analysis of haemoglobin variants using capillary zone electrophoresis
| العنوان: | Quantitative and qualitative analysis of haemoglobin variants using capillary zone electrophoresis |
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| المؤلفون: | Gerritsma, J, Sinnige, D, Drieze, C, Sittrop, B, Houtsma, P, Ulshorst-Jansen, N H, Huisman, W |
| المصدر: | Annals of Clinical Biochemistry: International Journal of Laboratory Medicine ; volume 37, issue 3, page 380-389 ; ISSN 0004-5632 1758-1001 |
| بيانات النشر: | SAGE Publications |
| سنة النشر: | 2000 |
| الوصف: | Capillary zone electrophoresis (CZE) has been introduced into the clinical chemistry laboratory because of its range of potential applications. In this paper, we evaluate an alkaline CZE method for the quantification of HbA 2 and HbF and also assess the combination of the alkaline CZE method with an acid CZE method for the determination of haemoglobin variants in an automated fashion. Correlation of the HbA 2 % determined between the HbA 2 -CZE method (alkaline conditions) and the Helena Sickle Thal Quick Column ® method was good ( r=0·91). The correlation between the HbF% determined by the HbA 2 -CZE method and by the alkaline denaturation method was acceptable ( r= 0·81). The HbA 2 -CZE method was able to identify a large number of haemoglobin variants. The variants HbC and HbE or HbS and HbD, however, had the same characteristics under alkaline conditions and could therefore not be discriminated from each other. The identification of these overlapping variants could be accomplished by the analysis of the blood specimens in combination with the HbA 1c -CZE method (acid conditions). We conclude that the presented applications for capillary zone electrophoresis can be used for quantitative and qualitative analysis of haemoglobin variants. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1258/0004563001899320 |
| الاتاحة: | https://doi.org/10.1258/0004563001899320 https://journals.sagepub.com/doi/pdf/10.1258/0004563001899320 |
| Rights: | https://journals.sagepub.com/page/policies/text-and-data-mining-license |
| رقم الانضمام: | edsbas.B0267B3E |
| قاعدة البيانات: | BASE |
| DOI: | 10.1258/0004563001899320 |
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