Academic Journal
Evaluation of the diagnostic performances of two commercially available assays for the detection of enteric adenovirus antigens
| العنوان: | Evaluation of the diagnostic performances of two commercially available assays for the detection of enteric adenovirus antigens |
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| المؤلفون: | Bonura F., Mascarella C., Filizzolo C., Bonura C., Ferraro D., Di Bernardo F., Collura A., Martella V., Giammanco G. M., De Grazia S. |
| المساهمون: | Bonura F., Mascarella C., Filizzolo C., Bonura C., Ferraro D., Di Bernardo F., Collura A., Martella V., Giammanco G.M., De Grazia S. |
| بيانات النشر: | Elsevier Inc. |
| سنة النشر: | 2021 |
| المجموعة: | IRIS Università degli Studi di Palermo |
| مصطلحات موضوعية: | Acute gastroenteriti, Chemiluminescent immunoassay, Diagnostic performance, Enteric adenoviruse, Fecal antigens detection, Immuno chromatography, Settore MED/07 - Microbiologia E Microbiologia Clinica |
| الوصف: | The performance of 2 antigenic commercial assays for enteric adenovirus (AdV) infection, bioNexia Rota-Adeno ImmunoChromatographic Tests (ICT) and LIAISON® Adenovirus ChemiLuminescence Immuno Assays (CLIA), was evaluated on 321 stools from children hospitalized for acute gastroenteritis in Palermo, Italy, using a Real time-PCR (Rt-PCR) as reference method. The CLIA showed higher sensitivity (77% vs 60%), accuracy (94.4 vs 90.9) and concordance (k: 0.81 vs 0.67) with respect to ICT, despite equivalent specificity (98.8%). Using the Ct values of the Rt-PCR as a proxy of the fecal viral load, similar Ct values (mean 9.32 vs 9.89) were observed among the true positive samples, whilst a significant difference (P < 0.05) was observed in false negative samples of CLIA (mean Ct 25.68) and ICT (mean Ct 19.87). Cross-reactivity with other enteric viruses was not observed. These results indicate that both the assays tested are suitable for diagnosis of AdV gastroenteritis. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/34280672; info:eu-repo/semantics/altIdentifier/wos/WOS:000704358300001; volume:101; issue:3; numberofpages:4; journal:DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE; http://hdl.handle.net/10447/519044 |
| DOI: | 10.1016/j.diagmicrobio.2021.115459 |
| الاتاحة: | http://hdl.handle.net/10447/519044 https://doi.org/10.1016/j.diagmicrobio.2021.115459 |
| Rights: | info:eu-repo/semantics/closedAccess |
| رقم الانضمام: | edsbas.AFFC3C5E |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.diagmicrobio.2021.115459 |
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