Academic Journal
The Arabidopsis DWARF27 gene encodes an all-trans-/9-cis-β-carotene isomerase and is induced by auxin, abscisic acid and phosphate deficiency
| العنوان: | The Arabidopsis DWARF27 gene encodes an all-trans-/9-cis-β-carotene isomerase and is induced by auxin, abscisic acid and phosphate deficiency |
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| المؤلفون: | Abuauf, Haneen W., Haider, Imran, Jia, Kunpeng, Ablazov, Abdugaffor, Mi, Jianing, Blilou, Ikram, Al-Babili, Salim |
| المساهمون: | Biological and Environmental Science and Engineering (BESE) Division, Bioscience Program, Center for Desert Agriculture, Plant Science |
| بيانات النشر: | Elsevier BV |
| سنة النشر: | 2018 |
| المجموعة: | King Abdullah University of Science and Technology: KAUST Repository |
| مصطلحات موضوعية: | Arabidopsis, abscisic acid, DWARF27, β-carotene isomerase, carotenoids, strigolactones |
| الوصف: | Strigolactones (SLs) are carotenoid-derived plant hormones that influence various aspects of plant growth and development in response to environmental conditions, especially nutrients deficiency. SLs are synthesized via a strict stereo-specific core pathway that leads to the intermediate carlactone, requiring the iron-containing polypeptide DWARF27 (D27) and the carotenoid cleavage dioxygenases 7 (CCD7) and 8 (CCD8). It has been shown that the rice OsD27 is a β-carotene isomerase catalyzing the interconversion of all-trans- into 9-cis-β -carotene. However, data about the enzymatic activity of D27 from other species are missing. Here, we investigated the activity and substrate specificity of the Arabidopsis AtD27 by testing a broad range of carotenoid substrates. Both in vivo and in vitro assays show that AtD27 catalyzes the reverse isomerization of all-trans-/9-cis-β-carotene. AtD27 did not isomerize 13-cis- or 15-cis-β-carotene, indicating high specificity for the C9-C10 double bond. The isomerization reaction was inhibited in the presence of silver acetate, pointing to the involvement of an iron-sulfur cluster. We further investigated the expression of AtD27, using Arabidopsis transgenic lines expressing β-glucuronidase (GUS) under the control of AtD27 native promoter. AtD27 is ubiquitously expressed throughout the plant with the highest expression in immature flowers. In lateral roots, AtD27 expression was induced by treatment with auxin and ABA, while the application of SL analogs did not show an effect. Lower ABA levels in atd27 mutant indicated an interference with the ABA pathway. Quantitative real-time RT-PCR showed that transcript levels of AtD27 and other SL biosynthetic genes in roots are induced upon phosphate starvation. Taken together, our study on AtD27 confirms the postulated enzymatic function of this enzyme, shows its strict substrate- and regio-specificity and indicates an important role in response to multiple plant hormones and phosphate deficiency. ; This work was supported by base line ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf; application/vnd.openxmlformats-officedocument.wordprocessingml.document |
| اللغة: | unknown |
| تدمد: | 0168-9452 |
| Relation: | https://www.sciencedirect.com/science/article/pii/S0168945218306022; Abuauf H, Haider I, Jia K-P, Ablazov A, Mi J, et al. (2018) The Arabidopsis DWARF27 gene encodes an all-trans-/9-cis-β-carotene isomerase and is induced by auxin, abscisic acid and phosphate deficiency. Plant Science. Available: http://dx.doi.org/10.1016/j.plantsci.2018.06.024.; Plant Science; http://hdl.handle.net/10754/628729 |
| DOI: | 10.1016/j.plantsci.2018.06.024 |
| الاتاحة: | http://hdl.handle.net/10754/628729 https://doi.org/10.1016/j.plantsci.2018.06.024 |
| Rights: | NOTICE: this is the author’s version of a work that was accepted for publication in Plant Science. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Plant Science, [, , (2018-09-11)] DOI:10.1016/j.plantsci.2018.06.024 . © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| رقم الانضمام: | edsbas.AFC58EAF |
| قاعدة البيانات: | BASE |
| تدمد: | 01689452 |
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| DOI: | 10.1016/j.plantsci.2018.06.024 |