Academic Journal
Delivery of siRNA to Ewing Sarcoma Tumor Xenografted on Mice, Using Hydrogenated Detonation Nanodiamonds: Treatment Efficacy and Tissue Distribution
| العنوان: | Delivery of siRNA to Ewing Sarcoma Tumor Xenografted on Mice, Using Hydrogenated Detonation Nanodiamonds: Treatment Efficacy and Tissue Distribution |
|---|---|
| المؤلفون: | Claveau, Sandra, Nehlig, Emilie, Garcia-Argote, Sébastien, Feuillastre, Sophie, Pieters, Grégory, Girard, Hugues, Arnault, Jean-Charles, Treussart, François, Bertrand, Jean-Rémi |
| المساهمون: | Aspects métaboliques et systémiques de l'oncogénèse pour de nouvelles approches thérapeutiques (METSY), Institut Gustave Roussy (IGR)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Laboratoire Lumière, Matière et Interfaces (LuMIn), CentraleSupélec-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Ecole Normale Supérieure Paris-Saclay (ENS Paris Saclay), Service de Chimie Bio-Organique et de Marquage (SCBM), Médicaments et Technologies pour la Santé (MTS), Université Paris-Saclay-Institut des Sciences du Vivant Frédéric JOLIOT (JOLIOT), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Paris-Saclay-Institut des Sciences du Vivant Frédéric JOLIOT (JOLIOT), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Laboratoire Capteurs Diamant (CEA, LIST) (LCD (CEA, LIST)), Département Métrologie Instrumentation & Information (CEA, LIST) (DM2I (CEA, LIST)), Laboratoire d'Intégration des Systèmes et des Technologies (LIST (CEA)), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Laboratoire d'Intégration des Systèmes et des Technologies (LIST (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, ANR-14-ENM2-0002,DiamESTar,Targeted delivery of therapeutic siRNA to Ewing sarcoma junction oncogene by traceable diamond nanocrystal – antibody fragment conjugate(2014) |
| المصدر: | ISSN: 2079-4991. |
| بيانات النشر: | HAL CCSD MDPI |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | biodistribution, delivery agent, in vivo delivery, Ewing sarcoma, drug delivery, siRNA, diamond, nanodiamond, cancer, anti-cancer therapeutic compound, hydrogenated detonation nanodiamond, tritium, labeled nanodiamond, nanomedicine, [SDV.IB]Life Sciences [q-bio]/Bioengineering |
| الوصف: | International audience ; Nanodiamonds of detonation origin are promising delivery agents of anti-cancer therapeutic compounds in a whole organism like mouse, owing to their versatile surface chemistry and ultra-small 5 nm average primary size compatible with natural elimination routes. However, to date, little is known about tissue distribution, elimination pathways and efficacy of nanodiamonds-based therapy in mice. In this report, we studied the capacity of cationic hydrogenated detonation nanodiamonds to carry active small interfering RNA (siRNA) in a mice model of Ewing sarcoma, a bone cancer of young adults due in the vast majority to the EWS-FLI1 junction oncogene. Replacing hydrogen gas by its radioactive analog tritium gas led to the formation of labeled nanodiamonds and allowed us to investigate their distribution throughout mouse organs and their excretion in urine and feces. We also demonstrated that siRNA directed against EWS-FLI1 inhibited this oncogene expression in tumor xenografted on mice. This work is a significant step to establish cationic hydrogenated detonation nanodiamond as an effective agent for in vivo delivery of active siRNA. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/32204428; PUBMED: 32204428; WOS: 000526090400153 |
| DOI: | 10.3390/nano10030553 |
| الاتاحة: | https://insu.hal.science/insu-02513366 https://insu.hal.science/insu-02513366v1/document https://insu.hal.science/insu-02513366v1/file/article_SandraClaveau_nanomaterials.pdf https://doi.org/10.3390/nano10030553 |
| Rights: | http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.ADABD195 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/nano10030553 |
|---|