Academic Journal
Optical imaging detects metabolic signatures associated with oocyte quality
| العنوان: | Optical imaging detects metabolic signatures associated with oocyte quality |
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| المؤلفون: | Tan, T.C.Y., Brown, H.M., Thompson, J.G., Mustafa, S., Dunning, K.R. |
| المصدر: | http://dx.doi.org/10.1093/biolre/ioac145. |
| بيانات النشر: | Oxford University Press (OUP) |
| سنة النشر: | 2022 |
| المجموعة: | The University of Adelaide: Digital Library |
| مصطلحات موضوعية: | autofluorescence, NAD(P)H, FAD, oocyte assessment, non-invasive, cellular metabolism, optical imaging, oxygen consumption rate, optical redox ratio |
| الوصف: | Oocyte developmental potential is intimately linked to metabolism. Existing approaches to measure metabolism in the cumulus oocyte complex (COC) do not provide information on the separate cumulus and oocyte compartments. Development of an assay that achieves this may lead to an accurate diagnostic for oocyte quality. Optical imaging of the autofluorescent cofactors reduced nicotinamide adenine dinucleotide (phosphate) [NAD(P)H] and flavin adenine dinucleotide (FAD) provides a spatially resolved indicator of metabolism via the optical redox ratio (FAD/[NAD(P)H + FAD]). This may provide an assessment of oocyte quality. Here, we determined whether the optical redox ratio is a robust methodology for measuring metabolism in the cumulus and oocyte compartments compared with oxygen consumption in the whole COC. We also determined whether optical imaging could detect metabolic differences associated with poor oocyte quality (etomoxir-treated). We used confocal microscopy to measure NAD(P)H and FAD, and extracellular flux to measure oxygen consumption. The optical redox ratio accurately reflected metabolism in the oocyte compartment when compared with oxygen consumption (whole COC). Etomoxir-treated COCs showed significantly lower levels of NAD(P)H and FAD compared to control. We further validated this approach using hyperspectral imaging, which is clinically compatible due to its low energy dose. This confirmed lower NAD(P)H and FAD in etomoxir-treated COCs. When comparing hyperspectral imaged vs nonimaged COCs, subsequent preimplantation development and post-transfer viability were comparable. Collectively, these results demonstrate that label-free optical imaging of metabolic cofactors is a safe and sensitive assay for measuring metabolism and has potential to assess oocyte developmental competence. ; Tiffany C. Y. Tan, Hannah M. Brown, Jeremy G. Thompson, Sanam Mustafa, and Kylie R. Dunning |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0006-3363 1529-7268 |
| Relation: | http://purl.org/au-research/grants/nhmrc/APP2003786; Biology of Reproduction, 2022; 107(4):1014-1025; https://hdl.handle.net/2440/136272; Brown, H.M. [0000-0001-6342-3316]; Thompson, J.G. [0000-0003-4941-7731]; Mustafa, S. [0000-0002-8677-5151]; Dunning, K.R. [0000-0002-0462-6479] |
| DOI: | 10.1093/biolre/ioac145 |
| الاتاحة: | https://hdl.handle.net/2440/136272 https://doi.org/10.1093/biolre/ioac145 |
| Rights: | © The Author(s) 2022. Published by Oxford University Press behalf of Society for the Study of Reproduction. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
| رقم الانضمام: | edsbas.AD4910E9 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 00063363 15297268 |
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| DOI: | 10.1093/biolre/ioac145 |