التفاصيل البيبلوغرافية
| العنوان: |
Additional file 6 of Revealing the composition of the eukaryotic microbiome of oyster spat by CRISPR-Cas Selective Amplicon Sequencing (CCSAS) |
| المؤلفون: |
Kevin Xu Zhong (5769752), Anna Cho (3217569), Christoph M. Deeg (6786839), Amy M. Chan (11758124), Curtis A. Suttle (11758127) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Microbiology, Genetics, Eukaryotic microbiome, 18S rRNA gene, Microeukaryote, CRISPR-Cas, Taxon-specific single-guide RNA, gRNA target site, CasOligo, CCSAS |
| الوصف: |
Additional file 6: Figure S3. Distribution of the number of gRNA-target-sites of each metazoan and plant species from the SILVA SSU database v119 [80]. These gRNA-target-site oligonucleotide sequences were identified, using the Cas9.gRNA.oligo1() algorithm, from the V4 region of the 18S rRNA gene that is flanked by the 18S "universal" primer set TAReuk454FWD1 / TAReukREV3 [54], and are used for designing and synthesizing the CRISPR-Cas9-compatible sgRNA. The taxon-specific gRNA-target-sites allows the design of the sgRNA to taxon-specifically cut the 18S rRNA gene sequence of a metazoan or plant host but not microeukaryotes (protists and fungi) using CRISPR-Cas9. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/journal_contribution/Additional_file_6_of_Revealing_the_composition_of_the_eukaryotic_microbiome_of_oyster_spat_by_CRISPR-Cas_Selective_Amplicon_Sequencing_CCSAS_/17084309 |
| DOI: |
10.6084/m9.figshare.17084309.v1 |
| الاتاحة: |
https://doi.org/10.6084/m9.figshare.17084309.v1 |
| Rights: |
CC BY + CC0 |
| رقم الانضمام: |
edsbas.AD3F66A3 |
| قاعدة البيانات: |
BASE |