Academic Journal
Fluorophore‐Conjugated Holliday Junctions for Generating Super‐Bright Antibodies and Antibody Fragments
| العنوان: | Fluorophore‐Conjugated Holliday Junctions for Generating Super‐Bright Antibodies and Antibody Fragments |
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| المؤلفون: | Li, Zeyang, Theile, Christopher S., Chen, Guan‐Yu, Bilate, Angelina M., Duarte, Joao N., Avalos, Ana M., Fang, Tao, Barberena, Roberto, Sato, Shuji, Ploegh, Hidde L. |
| المصدر: | Angewandte Chemie International Edition ; volume 54, issue 40, page 11706-11710 ; ISSN 1433-7851 1521-3773 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2015 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | The site‐specific modification of proteins with fluorophores can render a protein fluorescent without compromising its function. To avoid self‐quenching from multiple fluorophores installed in close proximity, we used Holliday junctions to label proteins site‐specifically. Holliday junctions enable modification with multiple fluorophores at reasonably precise spacing. We designed a Holliday junction with three of its four arms modified with a fluorophore of choice and the remaining arm equipped with a dibenzocyclooctyne substituent to render it reactive with an azide‐modified fluorescent single‐domain antibody fragment or an intact immunoglobulin produced in a sortase‐catalyzed reaction. These fluorescent Holliday junctions improve fluorescence yields for both single‐domain and full‐sized antibodies without deleterious effects on antigen binding. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1002/anie.201505277 |
| الاتاحة: | http://dx.doi.org/10.1002/anie.201505277 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fanie.201505277 https://onlinelibrary.wiley.com/doi/pdf/10.1002/anie.201505277 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/anie.201505277 |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.AD234A36 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/anie.201505277 |
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