Academic Journal
Curcumin-capped gold nanorods as optical sensing platform for sequence-specific detection of DNA based on their self-assembly
| العنوان: | Curcumin-capped gold nanorods as optical sensing platform for sequence-specific detection of DNA based on their self-assembly |
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| المؤلفون: | Mayuri, S, Jha, Niki S, Jha, S K |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2023 |
| المجموعة: | National Metallurgical Laboratory, Jamshedpur: Eprints@NML |
| مصطلحات موضوعية: | Nanoscience and Nanotechnology |
| الوصف: | We are reporting curcumin-induced gold nanorods as an optical sensing platform for the detection of sequence-specific DNA targets through their self-assembly. The combined effect of eco-friendly reducing agent (i.e., curcumin) and silver nitrate in a basic medium (i.e., pH 10) has been attributed to the formation of small gold nanorods (AuNRs) having approximate length and diameter i.e., 19.7 +/- 0.8 nm and 6.0 +/- 0.5 nm, respectively, and lower longitudinal surface plasmon resonance (SPR) enable to detect and analyze different biomarkers. Further, for evaluating cellular uptake of as-synthesized AuNRs, the cytotoxicity study has been carried out by 3(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay on A549 cells and HEPG2 cell lines, respectively, and shown approximately similar cytotoxicity. Interestingly, an as-synthesized optically and electronically active AuNRs-based nano biosensing platform enables to detection of sequence-specific DNA targets with a low level of detection limit i.e., LOD 8.6 +/- 0.15 pM for the complementary target (CT) DNA with higher sensitivity and better selectivity. Finally, this study suggests a simplistic bio-mediated approach to tuning the shape and size of AuNRs for sensitive, selective, and reliable nano-biosensing platforms for sequence-specific DNA detection related to cancer cells. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| Relation: | Mayuri, S and Jha, Niki S and Jha, S K (2023) Curcumin-capped gold nanorods as optical sensing platform for sequence-specific detection of DNA based on their self-assembly. International Journal of Biological Macromolecules, 253(Part 3) . |
| DOI: | 10.1016/j.ijbiomac.2023.126829 |
| الاتاحة: | http://eprints.nmlindia.org/9453/ https://10.1016/j.ijbiomac.2023.126829 |
| رقم الانضمام: | edsbas.AD1AB940 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.ijbiomac.2023.126829 |
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