Academic Journal
Investigation of the influence of high glucose on molecular and genetic responses: An in vitro study using a human intestine model
| العنوان: | Investigation of the influence of high glucose on molecular and genetic responses: An in vitro study using a human intestine model |
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| المؤلفون: | Boztepe, Tuğçe, Güleç, Şükrü |
| المساهمون: | Izmir Institute of Technology. Food Engineering |
| بيانات النشر: | BioMed Central Ltd. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | High glucose consumption, ABC transporter A1, Obesity, LCN15 gene, TXNIP gene, Caco-2 cell line |
| جغرافية الموضوع: | 10.1186/s12263-018-0602-x |
| الوصف: | Background: Dietary glucose consumption has increased worldwide. Long-term high glucose intake contributes to the development of obesity and type 2 diabetes mellitus (T2DM). Obese people tend to eat glucose-containing foods, which can lead to an addiction to glucose, increased glucose levels in the blood and intestine lumen, and exposure of intestinal enterocytes to high dietary glucose. Recent studies have documented a role for enterocytes in glucose sensing. However, the molecular and genetic relationship between high glucose levels and intestinal enterocytes has not been determined. We aimed to identify relevant target genes and molecular pathways regulated by high glucose in a well-established in vitro epithelial cell culture model of the human intestinal system (Caco-2 cells). Methods: Cells were grown in a medium containing 5.5 and 25 mM glucose in a bicameral culture system for 21 days to mimic the human intestine. Transepithelial electrical resistance was used to control monolayer formation and polarization of the cells. Total RNA was isolated, and genome-wide mRNA expression profiles were determined. Molecular pathways were analyzed using the DAVID bioinformatics program. Gene expression levels were confirmed by quantitative reverse transcription polymerase chain reaction (RT-qPCR). Results: Microarray gene expression data demonstrated that 679 genes (297 upregulated, 382 downregulated) were affected by high glucose treatment. Bioinformatics analysis indicated that intracellular protein export (p=0.0069) and ubiquitin-mediated proteolysis (p=0.024) pathways were induced, whereas glycolysis/gluconeogenesis (p<0.0001), pentose phosphate (p=0.0043), and fructose-mannose metabolism (p=0.013) pathways were downregulated, in response to high glucose. Microarray analysis of gene expression showed that high glucose significantly induced mRNA expression levels of thioredoxin-interacting protein (TXNIP, p=0.0001) and lipocalin 15 (LCN15, p=0.0016) and reduced those of ATP-binding cassette, sub-family A member ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1865-3499 |
| Relation: | Genes and Nutrition; info:eu-repo/grantAgreement/TUBITAK/KBAG/214Z217; Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı; Boztepe, T., and Güleç, Ş. (2018). Investigation of the influence of high glucose on molecular and genetic responses: An in vitro study using a human intestine model. Genes and Nutrition, 13(1). doi:10.1186/s12263-018-0602-x; https://doi.org/10.1186/s12263-018-0602-x; https://hdl.handle.net/11147/7568; 13; WOS:000432371900001; SCOPUS:2-s2.0-85046108118 |
| DOI: | 10.1186/s12263-018-0602-x |
| الاتاحة: | https://hdl.handle.net/11147/7568 https://doi.org/10.1186/s12263-018-0602-x |
| Rights: | open |
| رقم الانضمام: | edsbas.ACCD679E |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 18653499 |
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| DOI: | 10.1186/s12263-018-0602-x |