Academic Journal
Antigenic Stimuli do not Influence Thymic B Lymphocytes: A Morphological and Functional Study inGerm‐Free and Conventionally Reared Piglets
| العنوان: | Antigenic Stimuli do not Influence Thymic B Lymphocytes: A Morphological and Functional Study inGerm‐Free and Conventionally Reared Piglets |
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| المؤلفون: | Cukrowska, B., Trebichavský, I., Rossmann, P., Řeháková, Z., ŠInkora, J., Haverson, K., Lodinová-Žádníková, R., Tlaskalová-Hogenová, H. |
| المساهمون: | Ministerstvo Zdravotnictví Ceské Republiky, European Commission |
| المصدر: | Journal of Immunology Research ; volume 6, issue 3-4, page 171-178 ; ISSN 2314-8861 2314-7156 |
| بيانات النشر: | Wiley |
| سنة النشر: | 1997 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | We have recently reported that thymic B lymphocytes (TBL) are the first B‐cell subpopulation undergoing isotype switching to IgG and IgA during embryonic life. The aim of this study is to analyze the influence of antigenic stimulation on TBL location and activity using a germ‐free (GF) newborn pig model, in which maternal antibodies and antigens do not affect B‐cell development. Immunohistological analysis showed that TBL were disseminated mainly in the thymic medulla. There were no differences in the distribution of TBL, both in GF newborn piglets before and after colonization with Escherichia coli and in older conventionally reared (CONV) piglets. The number of immunoglobulin (Ig)‐secreting cells measured by the ELISPOT method was not influenced by microflora and food antigens. IgM‐positive cells secreting IgM and CD45RC‐positive cells spontaneously producing IgM, IgG, and IgA were detected in newborn thymus. Our findings suggest that TBL differentiation and Ig switching to IgG and IgA‐secreting cells is not influenced by external antigens and that the thymic microenviroment plays an important role in this process. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1155/1998/57820 |
| الاتاحة: | http://dx.doi.org/10.1155/1998/57820 http://downloads.hindawi.com/journals/jir/1998/057820.pdf https://onlinelibrary.wiley.com/doi/pdf/10.1155/1998/57820 |
| Rights: | http://creativecommons.org/licenses/by/3.0/ |
| رقم الانضمام: | edsbas.ACB0C871 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1155/1998/57820 |
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