التفاصيل البيبلوغرافية
| العنوان: |
Dauer larvae lacking 1–2 isoforms of daf-16 display col-19p::gfp more dimly and in fewer cells than daf-16(0) . |
| المؤلفون: |
Matthew J. Wirick (11708043), Allison R. Cale (11708046), Isaac T. Smith (11708049), Amelia F. Alessi (11708052), Margaret R. Starostik (11708055), Liberta Cuko (11708058), Kyal Lalk (11708061), Mikayla N. Schmidt (11708064), Benjamin S. Olson (9629251), Payton M. Salomon (11708067), Alexis Santos (11708070), Axel Schmitter-Sánchez (11708073), Himani Galagali (11708076), Kevin J. Ranke (11708079), Payton A. Wolbert (11708082), Macy L. Knoblock (11708085), John K. Kim (8575362), Xantha Karp (8729403) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Cell Biology, Genetics, Molecular Biology, Developmental Biology, Cancer, Infectious Diseases, Computational Biology, prematurely adopting differentiated, multipotent cell fate, misexpression phenotype observed, div >< p, binding protein orthologous, adverse environmental cues, single foxo ortholog, progenitor cells maintain, allow normal development, adult cell fate, stem cell maintenance, heterochronic gene network, caenorhabditis elegans <, dauer larvae via, >, promotes entry, heterochronic gene, elegans <, seam cells, dauer development, >/ foxo, normally adult, adult characteristics |
| الوصف: |
(A) Representative micrographs of dauer larvae scored in Fig 1D . In Fig 1D , any col-19p :: gfp expression in the lateral hypodermis was considered “on”, however there are qualitative differences in that expression in the different strains. Dashed lines in the control indicate the boundaries of the dauer larva, as determined by DIC optics. (B) Loss of 1–2 isoforms of daf-16 results in fewer cells expressing col-19p :: gfp compared to daf-16(0) dauer larvae. Quantification of the number of cells expressing col-19p :: gfp along one side of the lateral hypodermis between the pharynx and rectum. Each dot represents a dauer larva. *p = 0.0460 (Two-tailed Mann-Whitney Test). (C) Loss of 1–2 isoforms of daf-16 results in dimmer expression of col-19p :: gfp throughout the hypodermis compared to the daf-16(0) dauer larvae. Fluorescence levels were determined as described in S7 Fig . ****p<0.0001 (Two-tailed Mann-Whitney Test). Larvae shown in panels B-C were from an independent set of experiments from those in Fig 1D . n = 28–31; see S2 Table for complete underlying numerical data. (TIF) |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/Dauer_larvae_lacking_1_2_isoforms_of_i_daf-16_i_display_i_col-19p_gfp_i_more_dimly_and_in_fewer_cells_than_i_daf-16_0_i_/17017719 |
| DOI: |
10.1371/journal.pgen.1009881.s002 |
| الاتاحة: |
https://doi.org/10.1371/journal.pgen.1009881.s002 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.AC8832A1 |
| قاعدة البيانات: |
BASE |