Academic Journal
A cohort analysis of SARS-CoV-2 anti-spike protein receptor binding domain (RBD) IgG levels and neutralizing antibodies in fully vaccinated healthcare workers
| العنوان: | A cohort analysis of SARS-CoV-2 anti-spike protein receptor binding domain (RBD) IgG levels and neutralizing antibodies in fully vaccinated healthcare workers |
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| المؤلفون: | Padoan, Andrea, Cosma, Chiara, Della Rocca, Foscarina, Barbaro, Francesco, Santarossa, Claudia, Dall'Olmo, Luigi, Galla, Luisa, Cattelan, Annamaria, Cianci, Vito, Basso, Daniela, Plebani, Mario |
| المساهمون: | Padoan, Andrea, Cosma, Chiara, Della Rocca, Foscarina, Barbaro, Francesco, Santarossa, Claudia, Dall'Olmo, Luigi, Galla, Luisa, Cattelan, Annamaria, Cianci, Vito, Basso, Daniela, Plebani, Mario |
| بيانات النشر: | De Gruyter Open Ltd |
| سنة النشر: | 2022 |
| المجموعة: | Padua Research Archive (IRIS - Università degli Studi di Padova) |
| مصطلحات موضوعية: | 3 , months after second dose, BNT162b2, SARS-CoV-2, SARS-CoV-2 mRNA vaccine, antibodies kinetic, antibody, neutralizing antibodie, serology, vaccine third dose, Antibodies, Neutralizing, Viral, BNT162 Vaccine, COVID-19 Vaccine, Cohort Studie, Health Personnel, Human, Immunization, Secondary, Immunoglobulin G, COVID-19 |
| الوصف: | Objectives: The waning of humoral immunity after COVID-19 vaccine booster (third dose) has not yet been fully evaluated. This study updates data on anti-SARS-CoV-2 spike protein receptor binding domain (S-RBD) binding antibodies (bAb) and neutralizing antibodies (NAb) levels in individuals with homologous vaccination 3-4 months after receiving the booster dose. Methods: Fifty-five healthcare workers (HCW) from Padova University-Hospital were asked to collect serum samples for determining antibodies (Ab) at 12 (t12) and 28 (t28) days, at 6 months (t6m) after their first Comirnaty/BNT162b2 inoculation, and 3-4 months after receiving the 3rd homologous booster dose. HCW were monitored weekly for SARS-CoV-2 infection. Ab titers were measured by two chemiluminescent immunoassays, one targeting the S-RBD immunoglobulin G (IgG), and one surrogate viral neutralization test (sVNT), measuring NAb. Results: Twenty of the HCW had natural COVID-19 infection (COVID+) at different times, before either the first or the second vaccination. Median S-RBD IgG and NAb levels and their interquartile ranges 3-4 months after the 3rd dose were 1,076 (529-3,409) kBAU/L and 15.8 (11.3-38.3) mg/L, respectively, for COVID-, and 1,373 (700-1,373) kBAU/L and 21 (12.8-53.9) mg/L, respectively, for COVID+. At multivariate regression analyses, with age and gender included as covariates, S-RBD IgG bAb and sVNT NAb levels were closely associated with the time interval between serological determination and the 3rd vaccine dose (log10 βcoeff=-0.013, p=0.012 and log10 βcoeff=-0.010, p=0.025) for COVID+, whereas no such association was found in COVID-individuals. Conclusions: The third booster dose increases anti-SARS-CoV-2 Ab levels, elevated levels persisting for up to 3-4 months. Waning of Ab levels appears to be less pronounced for COVID+ individuals. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/35473824; info:eu-repo/semantics/altIdentifier/wos/WOS:000787681700001; volume:60; issue:7; firstpage:1110; lastpage:1115; numberofpages:6; journal:CLINICAL CHEMISTRY AND LABORATORY MEDICINE; http://hdl.handle.net/11577/3451525; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85129476614 |
| DOI: | 10.1515/cclm-2022-0322 |
| الاتاحة: | http://hdl.handle.net/11577/3451525 https://doi.org/10.1515/cclm-2022-0322 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.AC49475A |
| قاعدة البيانات: | BASE |
| DOI: | 10.1515/cclm-2022-0322 |
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