Academic Journal
Noninvasive Fluorine-19 Magnetic Resonance Relaxometry Measurement of the Partial Pressure of Oxygen in Acellular Perfluorochemical-loaded Alginate Microcapsules Implanted in the Peritoneal Cavity of Nonhuman Primates
| العنوان: | Noninvasive Fluorine-19 Magnetic Resonance Relaxometry Measurement of the Partial Pressure of Oxygen in Acellular Perfluorochemical-loaded Alginate Microcapsules Implanted in the Peritoneal Cavity of Nonhuman Primates |
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| المؤلفون: | Safley, Susan A., Graham, Melanie L., Weegman, Bradley P., Einstein, Samuel A., Barber, Graham F., Janecek, Jody J., Mutch, Lucas A., Singh, Amar, Ramachandran, Sabarinathan, Garwood, Michael, Sambanis, Athanassios, Papas, Klearchos K., Hering, Bernhard J., Weber, Collin J. |
| المصدر: | Transplantation ; volume 104, issue 2, page 259-269 ; ISSN 0041-1337 |
| بيانات النشر: | Ovid Technologies (Wolters Kluwer Health) |
| سنة النشر: | 2020 |
| الوصف: | Background. We have utilized a noninvasive technique for measuring the partial pressure of oxygen (pO 2 ) in alginate microcapsules implanted intraperitoneally in healthy nonhuman primates (NHPs). Average pO 2 is important for determining if a transplant site and capsules with certain passive diffusion characteristics can support the islet viability, metabolic activity, and dose necessary to reverse diabetes. Methods. Perfluoro-15-crown-5-ether alginate capsules were infused intraperitoneally into 3 healthy NHPs. Peritoneal pO 2 levels were measured on days 0 and 7 using fluorine-19 magnetic resonance relaxometry and a fiber-optic probe. Fluorine-19 MRI was used to determine the locations of capsules within the peritoneal space on days 0 and 7. Gross and histologic evaluations of the capsules were used to assess their biocompatibility postmortem. Results. At day 0 immediately after infusion of capsules equilibrated to room air, capsules were concentrated near the infusion site, and the pO 2 measurement using magnetic resonance relaxometry was 147 ± 9 mm Hg. On day 7 after capsules were dispersed throughout the peritoneal cavity, the pO 2 level was 61 ± 11 mm Hg. Measurements using the fiber-optic oxygen sensor were 132 ± 7.5 mm Hg (day 0) and 89 ± 6.1 mm Hg (day 7). Perfluoro-15-crown-5-ether capsules retrieved on day 7 were intact and free-floating without host cell attachment, although the numbers of peritoneal CD20 + B cells, CD4 + and CD8 + T cells, and CD14 + macrophages increased consistent with a mild foreign body reaction. Conclusions. The peritoneal pO 2 of normal NHPs is relatively low and we predict would decrease further when encapsulated islets are transplanted intraperitoneally. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1097/tp.0000000000002896 |
| DOI: | 10.1097/TP.0000000000002896 |
| الاتاحة: | http://dx.doi.org/10.1097/tp.0000000000002896 https://journals.lww.com/10.1097/TP.0000000000002896 |
| رقم الانضمام: | edsbas.AB91F9C2 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1097/tp.0000000000002896 |
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