Academic Journal
Genome organization of grapevine fanleaf nepovirus RNA2 deduced from the 122K polyprotein P2 in vitro cleavage products
| العنوان: | Genome organization of grapevine fanleaf nepovirus RNA2 deduced from the 122K polyprotein P2 in vitro cleavage products |
|---|---|
| المؤلفون: | Rogrio Margis, Christophe Ritzenthaler, Joseph Reinbolt, Monique Pinck, Lothaire Pinck |
| المساهمون: | The Pennsylvania State University CiteSeerX Archives |
| المصدر: | http://vir.sgmjournals.org/content/74/9/1919.full.pdf. |
| سنة النشر: | 1993 |
| المجموعة: | CiteSeerX |
| الوصف: | The full-length transcript of grapevine fanleaf virus (GFLV) RNA2 produces a primary product of 122K when translated in the rabbit reticulocyte system. This 122K polyprotein is completely processed in vitro by the RNAl-encoded 24K proteinase. The positions of the cleavage sites within the polyprotein have been mapped and the genome organization of GFLV-F13 RNA2 has been established. The order of mature proteins in the 122K polyprotein is the amino-terminal 28K protein, the 38K protein followed by the 56K coat protein at the carboxy terminus. These proteins represent the final cleavage products of the 122K polyprotein. A 66K protein which yields 28K and 38K proteins constitutes the major maturation intermediate. Microsequencing of the amino extremity of radioactively labelled 38K protein allowed identification of the Cys257/Ala 258 site as the cleavage site recognized by the GFLV proteinase between the 28K and the 38K proteins in the 66K protein in addition to the Arg6°5/Gly ~°6 site between the 38K protein and the coat protein. |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.323.3817; http://vir.sgmjournals.org/content/74/9/1919.full.pdf |
| الاتاحة: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.323.3817 http://vir.sgmjournals.org/content/74/9/1919.full.pdf |
| Rights: | Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| رقم الانضمام: | edsbas.AB19D636 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |