التفاصيل البيبلوغرافية
| العنوان: |
A Nanometric Probe of the Local Proton Concentration in Microtubule-Based Biophysical Systems |
| المؤلفون: |
Aarat P. Kalra (11882763), Boden B. Eakins (10432625), Sergei I. Vagin (1715734), Hui Wang (30400), Sahil D. Patel (10432628), Philip Winter (291799), Maral Aminpour (3596306), John D. Lewis (174673), Vahid Rezania (3129525), Karthik Shankar (1948327), Gregory D. Scholes (1272651), Jack A. Tuszynski (160860), Bernhard Rieger (1443451), Alkiviathes Meldrum (6021191) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Cell Biology, Biotechnology, Cancer, Space Science, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Physical Sciences not elsewhere classified, localized ionic condensation, based biophysical systems, local proton concentration, local cytoplasmic ph, conventional sensing techniques, show experimentally due, microtubules concentrate protons, sensing concept, protonic concentration, ph scale, thus allowing, subtle changes, results confirm, protein polymers, profound impact, one unit, nanometric probe, intracellular structures, functional sensor, direct measurement, cell via |
| الوصف: |
We show a double-functional fluorescence sensing paradigm that can retrieve nanometric pH information on biological structures. We use this method to measure the extent of protonic condensation around microtubules, which are protein polymers that play many roles crucial to cell function. While microtubules are believed to have a profound impact on the local cytoplasmic pH, this has been hard to show experimentally due to the limitations of conventional sensing techniques. We show that subtle changes in the local electrochemical surroundings cause a double-functional sensor to transform its spectrum, thus allowing a direct measurement of the protonic concentration at the microtubule surface. Microtubules concentrate protons by as much as one unit on the pH scale, indicating a charge storage role within the cell via the localized ionic condensation. These results confirm the bioelectrical significance of microtubules and reveal a sensing concept that can deliver localized biochemical information on intracellular structures. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/journal_contribution/A_Nanometric_Probe_of_the_Local_Proton_Concentration_in_Microtubule-Based_Biophysical_Systems/17698122 |
| DOI: |
10.1021/acs.nanolett.1c04487.s001 |
| الاتاحة: |
https://doi.org/10.1021/acs.nanolett.1c04487.s001 |
| Rights: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.AAB07CD2 |
| قاعدة البيانات: |
BASE |