Academic Journal
Membrane stretch increases the activity of Ca2+ -activated K+ channels in rabbit coronary vascular smooth muscles
| العنوان: | Membrane stretch increases the activity of Ca2+ -activated K+ channels in rabbit coronary vascular smooth muscles |
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| المساهمون: | Cheol Joo Lee, Sung Choon kwon, Young Ho Lee, Duck Sun Ahn, Bok Soon Kang, Ahn, Duk Sun |
| بيانات النشر: | Yonsei University |
| سنة النشر: | 2000 |
| مصطلحات موضوعية: | Animals, Arachidonic Acid/pharmacology, Calcium/metabolism, Calcium/pharmacology, Cell Membrane/physiology, Coronary Vessels/physiology, Hypotonic Solutions/pharmacology, Membrane Potentials, Muscle, Smooth, Vascular/physiology, Potassium Channels/physiology, Rabbits, Stretch, Ca2-activated K channel, coronary smooth muscle, hypotonic solution, arachidonic acid, albumin |
| الوصف: | It has been proposed that Ca(2+)-activated K+ channels play an essential role in maintaining vascular tone during stretch of blood vessel. However, the underlying mechanism of stretch-induced change of Ca(2+)-activated K+ channel activities are still unknown. The present experiment was designed to investigate the effect of membrane stretch on these channels whose activity was measured from rabbit coronary smooth muscle cells using a patch clamp technique. Ca(2+)-activated K+ channel were identified by their Ca2+ and voltage dependencies and its large conductances as in other preparations. Perfusion of cells with a hypotonic solution, which mimics stretching the cell membrane by making a cell swelling, produced an increase in channel activity in cell-attached patch mode. The similar increase was observed when negative pressure was applied into the patch pipette for stretching the cell membrane within a patch area. In inside-out patch, stretch still increased channel activity even under the conditions which exclude the possible involvement of secondary messengers, or of transmembrane Ca2+ influx via stretch-activated cation channels. Pretreatment of arachidonic acid or albumin showed no effect on stretch-induced channel activation, excluding the possibility of fatty acids mediated channel activation during membrane stretch. These results indicate that the stretch may directly increase the activity of Ca(2+)-activated K+ channels in our experimental condition. ; open |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0513-5796 1976-2437 |
| Relation: | Yonsei Medical Journal; J02813; OAK-2000-00621; https://ir.ymlib.yonsei.ac.kr/handle/22282913/171887; T200001420; Yonsei Medical Journal, Vol.41(2) : 266-272, 2000 |
| DOI: | 10.3349/ymj.2000.41.2.266 |
| الاتاحة: | https://ir.ymlib.yonsei.ac.kr/handle/22282913/171887 https://doi.org/10.3349/ymj.2000.41.2.266 |
| Rights: | CC BY-NC-ND 2.0 KR |
| رقم الانضمام: | edsbas.AAA7D84E |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 05135796 19762437 |
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| DOI: | 10.3349/ymj.2000.41.2.266 |