التفاصيل البيبلوغرافية
| العنوان: |
DataSheet_1_Humanized MISTRG as a preclinical in vivo model to study human neutrophil-mediated immune processes.docx |
| المؤلفون: |
Paula Martinez-Sanz, Adrien R. G. Laurent, Edith Slot, Mark Hoogenboezem, Nikolina Bąbała, Robin van Bruggen, Anthony Rongvaux, Richard A. Flavell, Godelieve A. M. Tytgat, Katka Franke, Hanke L. Matlung, Taco W. Kuijpers, Derk Amsen, Julien J. Karrich |
| سنة النشر: |
2023 |
| المجموعة: |
Frontiers: Figshare |
| مصطلحات موضوعية: |
Immunology, Applied Immunology (incl. Antibody Engineering, Xenotransplantation and T-cell Therapies), Autoimmunity, Cellular Immunology, Humoural Immunology and Immunochemistry, Immunogenetics (incl. Genetic Immunology), Innate Immunity, Transplantation Immunology, Tumour Immunology, Immunology not elsewhere classified, Genetic Immunology, Animal Immunology, Veterinary Immunology, neutrophils, animal model, humanized immune system mouse, next generation humanized mouse models, preclinical study, MISTRG |
| الوصف: |
Introduction MISTRG mice have been genetically modified to allow development of a human myeloid compartment from engrafted human CD34+ haemopoietic stem cells, making them particularly suited to study the human innate immune system in vivo. Here, we characterized the human neutrophil population in these mice to establish a model that can be used to study the biology and contribution in immune processes of these cells in vivo. Methods and results We could isolate human bone marrow neutrophils from humanized MISTRG mice and confirmed that all neutrophil maturation stages from promyelocytes (CD11b–CD16–) to end-stage segmented cells (CD11b+CD16+) were present. We documented that these cells possessed normal functional properties, including degranulation, reactive oxygen species production, adhesion, and antibody-dependent cellular cytotoxicity towards antibody-opsonized tumor cells ex vivo. The acquisition of functional capacities positively correlated with the maturation state of the cell. We found that human neutrophils were retained in the bone marrow of humanized MISTRG mice during steady state. However, the mature segmented CD11b+CD16+ human neutrophils were released from the bone marrow in response to two well-established neutrophil-mobilizing agents (i.e., G-CSF and/or CXCR4 antagonist Plerixafor). Moreover, the neutrophil population in the humanized MISTRG mice actively reacted to thioglycolate-induced peritonitis and could infiltrate implanted human tumors, as shown by flow cytometry and fluorescent microscopy. Discussion These results show that functional human neutrophils are generated and can be studied in vivo using the humanized MISTRG mice, providing a model to study the various functions of neutrophils in inflammation and in tumors. |
| نوع الوثيقة: |
dataset |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/dataset/DataSheet_1_Humanized_MISTRG_as_a_preclinical_in_vivo_model_to_study_human_neutrophil-mediated_immune_processes_docx/22231012 |
| DOI: |
10.3389/fimmu.2023.1105103.s001 |
| الاتاحة: |
https://doi.org/10.3389/fimmu.2023.1105103.s001
https://figshare.com/articles/dataset/DataSheet_1_Humanized_MISTRG_as_a_preclinical_in_vivo_model_to_study_human_neutrophil-mediated_immune_processes_docx/22231012 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.AA6BE78D |
| قاعدة البيانات: |
BASE |