التفاصيل البيبلوغرافية
| العنوان: |
Validation of the X-ray microtomography in the assessment of duodenal morphometry and surface area in celiac disease |
| المؤلفون: |
Virta, Johannes, Hannula, Markus, Lindfors, Katri, Tamminen, Ilmari, Taavela, Juha, Huhtala, Heini, Kaukinen, Katri, Saavalainen, Päivi, Hyttinen, Jari, Kurppa, Kalle |
| المساهمون: |
Immunomics, Department of Medical and Clinical Genetics |
| بيانات النشر: |
Frontiers Media |
| سنة النشر: |
2023 |
| المجموعة: |
Helsingfors Universitet: HELDA – Helsingin yliopiston digitaalinen arkisto |
| مصطلحات موضوعية: |
celiac disease, biopsies, histology, diagnosis, micro-CT, imaging, VILLOUS ATROPHY, REPRODUCIBILITY, General medicine, internal medicine and other clinical medicine, Surgery, anesthesiology, intensive care, radiology |
| الوصف: |
BackgroundDuodenal histology remains the diagnostic reference standard in celiac disease. However, traditional methods have suboptimal sensitivity and reproducibility for early mucosal changes and research purposes. We validated a recently introduced micro-CT imaging method for an accurate digital evaluation of duodenal histomorphometry and mucosal surface areas. MethodsEndoscopic biopsies from 58 individuals were utilized for the micro-CT imaging, selecting histological changes ranging from normal to severely damaged mucosa. The imaging protocol was optimized for practicability and resolution. The Bland-Altman method was applied to test intra- and interobserver variations in the blinded measurements. ResultsThe 3D micro-CT reconstructions enabled easy and precise digital cutting with optimal orientation and computer-assisted measurement of the surface area. Intraobserver analysis of morphological measurements showed a mean difference of 0.011 with limits of agreement (LA) from -0.397 to 0.375 and a standard deviation (SD) of 0.197. The corresponding figures for interobserver analysis were 0.080, from -0.719 to 0.537 and 0.320, respectively. The intraclass correlation coefficients (ICC) for the intraobserver and interobserver variations were 0.981 and 0.954, respectively. Intraobserver surface area analysis yielded a mean difference of 0.010, LA from -0.764 to 0.785 and an SD of 0.395, and an interobserver analysis mean difference of 0.028, LA from -0.642 to 0.698 and SD of 0.342. The respective ICCs for the intra- and interobserver variations were 0.963 and 0.972. ConclusionsMicro-CT showed excellent accuracy and reproducibility in the evaluation of mucosal morphometry and surface areas. The improved sensitivity for histological changes is a powerful tool for the diagnosis of celiac disease and for clinical and pharmacological studies. ; Peer reviewed |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
The work was funded by the Academy of Finland, the Finnish Funding Agency for Technology and Innovation, the Sigrid Juselius Foundation, the Foundation for Pediatric Research, the Paeivikki and Sakari Sohlberg Foundation, the University Consortium of Seinaejoki, and the Competitive State Research Financing of the Expert Area of Tampere University Hospital. The funders had no role in the design or conduct of the study.; Virta , J , Hannula , M , Lindfors , K , Tamminen , I , Taavela , J , Huhtala , H , Kaukinen , K , Saavalainen , P , Hyttinen , J & Kurppa , K 2022 , ' Validation of the X-ray microtomography in the assessment of duodenal morphometry and surface area in celiac disease ' , Frontiers in Immunology , vol. 13 , 945197 . https://doi.org/10.3389/fimmu.2022.945197; http://hdl.handle.net/10138/353503; baf4ab60-c082-4ec9-92c6-dd785e38b7ed; 000874001600001 |
| الاتاحة: |
http://hdl.handle.net/10138/353503 |
| Rights: |
cc_by ; info:eu-repo/semantics/openAccess ; openAccess |
| رقم الانضمام: |
edsbas.A9EB6CCC |
| قاعدة البيانات: |
BASE |