Academic Journal
Activity-enhanced DNAzyme for design of label-free copper( ii ) biosensor
| العنوان: | Activity-enhanced DNAzyme for design of label-free copper( ii ) biosensor |
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| المؤلفون: | Etheridge, William, Brossard, Frederic, Zheng, Sitan, Moench, Svenja, Pavagada, Suraj, Owens, Róisín M., Fruk, Ljiljana |
| المصدر: | Nanoscale, 15 (25), 10776–10782 ; ISSN: 2040-3364, 2040-3372 |
| بيانات النشر: | Royal Society of Chemistry |
| سنة النشر: | 2023 |
| المجموعة: | KITopen (Karlsruhe Institute of Technologie) |
| مصطلحات موضوعية: | ddc:570, Life sciences, biology, info:eu-repo/classification/ddc/570 |
| الوصف: | Metal ion-driven, DNA-cleaving DNAzymes are characterised by high selectivity and specificity. However, their use for metal ion sensing remains largely unexplored due to long reaction times and poor reaction yields relative to RNA-cleaving DNAzymes and other sensing strategies. Herein we present a study demonstrating a significant rate enhancement of a copper-selective DNA cleaving DNAzyme by both polydopamine (PDA) and gold (Au) nanoparticles (NPs). PDA NPs enhance the reaction through the production of hydrogen peroxide, while for AuNPs the enhancement is aided by the presence of citrate surface moeities, both of which drive the oxidative cleavage of the substrate. A 50-fold enhancement for PDA NPs makes the combination of PDA and DNAzyme suitable for a practical application as a sensitive biosensor for Cu(II) ions. Using DNAzyme deposition onto a gold electrode followed by Polydopamine Assisted DNA Immobilisation (PADI), we achieve a cost-effective, label-free and fast (within 15 min) electrochemical biosensor with a limit of detection of 180 nmol (11 ppm), thus opening a route for the rational design of a new generation of hybrid DNAzyme-based biosensors. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/wos/001013690700001; info:eu-repo/semantics/altIdentifier/issn/2040-3364; info:eu-repo/semantics/altIdentifier/issn/2040-3372; https://publikationen.bibliothek.kit.edu/1000160711; https://publikationen.bibliothek.kit.edu/1000160711/151033123; https://doi.org/10.5445/IR/1000160711 |
| DOI: | 10.5445/IR/1000160711 |
| الاتاحة: | https://publikationen.bibliothek.kit.edu/1000160711 https://publikationen.bibliothek.kit.edu/1000160711/151033123 https://doi.org/10.5445/IR/1000160711 |
| Rights: | https://creativecommons.org/licenses/by/3.0/deed.de ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.A93E4976 |
| قاعدة البيانات: | BASE |
| DOI: | 10.5445/IR/1000160711 |
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