Academic Journal
Comparison of PCR methods for detecting fetal RhDin maternal plasma
| العنوان: | Comparison of PCR methods for detecting fetal RhDin maternal plasma |
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| المؤلفون: | Atamaniuk, Johanna, Stuhlmeier, Karl M., Karimi, Alireza, Mueller, Mathias M. |
| المصدر: | Journal of Clinical Laboratory Analysis ; volume 23, issue 1, page 24-28 ; ISSN 0887-8013 1098-2825 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2009 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Background : Aim of this study was to establish the method yielding the highest sensitivity routinely used to determine fetal RhD type and gender from maternal cell‐free plasma DNA in different periods of gestation. Methods : Plasma DNA concentrations were measured from 46 pregnant women in different gestational periods and tested for RhD using three different PCR methods on exon 7: Thermal Cycler, Taqman method on LightCycler, and melting curve analysis on LightCycler. In addition, fetal gender was determined by PCR. Cell‐free plasma DNA was measured in 100 healthy volunteers as a reference group. Results : The mean value of cell‐free plasma DNA in the reference group was 10.9 pg/µL mean, (standard deviation (SD): 3.66) in 50 healthy women and 12.7 pg/µL (SD: 8.2) in 50 healthy men. In the firsttrimester of pregnancy cell‐free plasmaDNA was 14.9 pg/µL mean, (SD: 4.2), in the second trimester 15.4 pg/µL mean, (SD: 4.96), and the maximum was achieved in the third trimester of pregnancy 15.6 pg/µl mean, (SD: 6.49). TaqMan probes had the same accuracy, when compared with Thermal Cycler technology (46 samples, 6 failures). Using real‐time PCR with melting curve analysis 12 of 17 samples were correctly tested. Gender determination was correctly in 41 of 46 samples. Conclusion : RhD determinations with TaqMan and Thermal Cycler technology are useful methods for fetal RhD prediction. To increase the accuracy of RhD determination it is necessary to test on other exons in addition. J. Clin. Lab. Anal. 23:24–28, 2009. © 2009 Wiley‐Liss, Inc. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1002/jcla.20282 |
| الاتاحة: | http://dx.doi.org/10.1002/jcla.20282 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjcla.20282 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jcla.20282 |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.A8D6AF76 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/jcla.20282 |
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