Academic Journal

Activation of mutated TRPA1 ion channel by resveratrol in human prostate cancer associated fibroblasts (CAF)

التفاصيل البيبلوغرافية
العنوان: Activation of mutated TRPA1 ion channel by resveratrol in human prostate cancer associated fibroblasts (CAF)
المؤلفون: Vancauwenberghe, Eric, Noyer, Lucile, Derouiche, Sandra, Lemonnier, Loïc, Gosset, Pierre, Sadofsky, Laura, Mariot, Pascal, Warnier, Marine, Bokhobza, Alexandre, Slomianny, Christian, Mauroy, Brigitte, Bonnal, Jean‐louis, Dewailly, Etienne, Delcourt, Philippe, Allart, Laurent, Desruelles, Emilie, Prevarskaya, Natalia, Roudbaraki, Morad
المساهمون: LabEx ICST, Laboratoire d'excellence Canaux Ioniques d'Intérêt Thérapeutique, Equipe Labélisée par la Ligue Nationale Contre le Cancer, SIRIC ONCOLille, Université de Lille, Sciences et Technologies, Laboratoire de Physiologie Cellulaire - U 1003 (PHYCELL), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille, Groupement des Hôpitaux de l'Institut Catholique de Lille (GHICL), Université catholique de Lille (UCL), Rôle des canaux ioniques membranaires et du calcium intracellulaire dans la physiopathologie de la prostate, Université de Lille, Sciences et Technologies-Institut National de la Santé et de la Recherche Médicale (INSERM)
المصدر: ISSN: 0899-1987.
بيانات النشر: HAL CCSD
Wiley
سنة النشر: 2017
المجموعة: LillOA (HAL Lille Open Archive, Université de Lille)
مصطلحات موضوعية: [SDV]Life Sciences [q-bio]
الوصف: International audience ; Previous studies showed the effects of resveratrol (RES) on several cancer cells, including prostate cancer (PCa) cell apoptosis without taking into consideration the impact of the tumor microenvironment (TME). The TME is composed of cancer cells, endothelial cells, blood cells, and cancer‐associated fibroblasts (CAF), the main source of growth factors. The latter cells might modify in the TME the impact of RES on tumor cells via secreted factors. Recent data clearly show the impact of CAF on cancer cells apoptosis resistance via secreted factors. However, the effects of RES on PCa CAF have not been studied so far. We have investigated here for the first time the effects of RES on the physiology of PCa CAF in the context of TME. Using a prostate cancer CAF cell line and primary cultures of CAF from prostate cancers, we show that RES activates the N‐terminal mutated Transient Receptor Potential Ankyrin 1 (TRPA1) channel leading to an increase in intracellular calcium concentration and the expression and secretion of growth factors (HGF and VEGF) without inducing apoptosis in these cells. Interestingly, in the present work, we also show that when the prostate cancer cells were co‐cultured with CAF, the RES‐induced cancer cell apoptosis was reduced by 40%, an apoptosis reduction canceled in the presence of the TRPA1 channel inhibitors. The present work highlights CAF TRPA1 ion channels as a target for RES and the importance of the channel in the epithelial‐stromal crosstalk in the TME leading to resistance to the RES‐induced apoptosis.
نوع الوثيقة: article in journal/newspaper
اللغة: English
Relation: hal-04210362; https://hal.science/hal-04210362; https://hal.science/hal-04210362/document; https://hal.science/hal-04210362/file/EMB%202017-03-16%2014581%20Sadofsky.pdf
DOI: 10.1002/mc.22642
الاتاحة: https://hal.science/hal-04210362
https://hal.science/hal-04210362/document
https://hal.science/hal-04210362/file/EMB%202017-03-16%2014581%20Sadofsky.pdf
https://doi.org/10.1002/mc.22642
Rights: info:eu-repo/semantics/OpenAccess
رقم الانضمام: edsbas.A7CF4F1F
قاعدة البيانات: BASE