Academic Journal
Evaluation of Cold Plasma Treatment and Safety in Disinfecting 3-week Root Canal Enterococcus faecalis Biofilm In Vitro
| العنوان: | Evaluation of Cold Plasma Treatment and Safety in Disinfecting 3-week Root Canal Enterococcus faecalis Biofilm In Vitro |
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| المؤلفون: | Li, Yinglong, Sun, Ke, Ye, Guopin, Liang, Yongdong, Pan, Hong, Wang, Guomin, Zhao, Yijiao, Pan, Jie, Zhang, Jue, Fang, Jing |
| المساهمون: | Pan, J (reprint author), Peking Univ, Sch & Hosp Stomatol, Dept Gen Dent, 22 Zhongguancun South St, Beijing 100081, Peoples R China., Peking Univ, Acad Adv Interdisciplinary Studies, Dept Gen Dent, Beijing 100081, Peoples R China., Peking Univ, Coll Engn, Beijing 100081, Peoples R China., Peking Univ, Sch & Hosp Stomatol, Natl Engn Lab Digital & Mat Technol Stomatol, Beijing 100081, Peoples R China., Peking Univ, Sch & Hosp Stomatol, Dept Gen Dent, Beijing 100081, Peoples R China., Peking Univ, Sch & Hosp Stomatol, Dept Gen Dent, 22 Zhongguancun South St, Beijing 100081, Peoples R China. |
| المصدر: | PubMed ; SCI |
| بيانات النشر: | JOURNAL OF ENDODONTICS |
| سنة النشر: | 2015 |
| المجموعة: | Peking University Institutional Repository (PKU IR) / 北京大学机构知识库 |
| مصطلحات موضوعية: | Cold plasma, Enterococcus faecalis biofilm, mechanical, safety, microhardness, roughness, 2-PERCENT CHLORHEXIDINE GEL, CALCIUM HYDROXIDE, ANTIMICROBIAL EFFICACY, BACTERIAL BIOFILMS, SUSCEPTIBILITY, INFECTION, MODEL, INSTRUMENTATION, RESISTANCE, TRIAL |
| الوصف: | Introduction: Although endodontic infection is caused by multi-bacteria species, Enterococcus faecalis is usually isolated in chronic apical periodontitis. The aim of this study was to evaluate the effectiveness and mechanical safety of cold plasma therapy in disinfecting 3-week E. faecalis biofilms. Methods: Teeth with 3-week E. faecalis biofilm were treated with AC argon/oxygen (Ar/O-2) cold plasma for various treatment times and compared with those treated with Ca(OH)(2), 2% chlorhexidine gel, and Ca(OH)(2)/chlorhexidine for a week. Antimicrobial efficacy was assessed by colony-forming unit method. Scanning electron microscopy was used to assess the morphologic changes of E. faecalis biofilm by plasma. Confocal laser scanning microscopy was used to confirm the viability of the biofilm after the plasma treatment. Microhardness and roughness changes of root canal dentin caused by plasma were verified with Vickers Hardness Tester and 3D Profile Measurement Laser Microscope, respectively. Results: There were no detectable live bacteria after 12 minutes of cold plasma treatment. This was further confirmed by scanning electron microscopy and confocal laser scanning microscopy results. Microhardness and roughness of root canal dentin showed no significant difference after plasma treatment. Conclusions: Atmospheric pressure cold plasma is an effective therapy in endodontics for its strong sterilization effect on fully matured biofilm within a few minutes. Meanwhile, it has art accepted mechanical safety for its low temperature and not affecting the microhardness and roughness of root canal dentin significantly. ; 985 program of Peking University ; SCI(E) ; PubMed ; ARTICLE ; panjie72@sina.com ; 8 ; 1325-1330 ; 41 |
| نوع الوثيقة: | journal/newspaper |
| اللغة: | English |
| تدمد: | 0099-2399 1878-3554 |
| Relation: | JOURNAL OF ENDODONTICS.2015,41,(8),1325-1330.; 1296196; http://hdl.handle.net/20.500.11897/417702; WOS:000359183600020 |
| DOI: | 10.1016/j.joen.2014.10.020 |
| الاتاحة: | https://hdl.handle.net/20.500.11897/417702 https://doi.org/10.1016/j.joen.2014.10.020 |
| رقم الانضمام: | edsbas.A787264B |
| قاعدة البيانات: | BASE |
| تدمد: | 00992399 18783554 |
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| DOI: | 10.1016/j.joen.2014.10.020 |