Academic Journal
Rapid Detection of Candida albicans in Clinical Blood Samples by Using a TaqMan-Based PCR Assay
| العنوان: | Rapid Detection of Candida albicans in Clinical Blood Samples by Using a TaqMan-Based PCR Assay |
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| المؤلفون: | Maaroufi, Younes, Heymans, Corine, De Bruyne, Jean-Marc, Duchateau, Valerie, Rodriguez-Villalobos, Hector, Aoun, Michel, Crokaert, Françoise |
| المصدر: | Journal of Clinical Microbiology ; volume 41, issue 7, page 3293-3298 ; ISSN 0095-1137 1098-660X |
| بيانات النشر: | American Society for Microbiology |
| سنة النشر: | 2003 |
| الوصف: | We describe a rapid and reproducible PCR assay for quantitation of the Candida albicans ribosomal DNA (rDNA) in clinical blood samples based on the TaqMan principle (Applied Biosystems), in which a signal is generated by cleavage of a template-specific probe during amplification. We used two fluorogenic probes based on universal, fungus-specific primers, one for the detection of C. albicans species DNA and one for the detection of all Candida genus DNA. C. albicans blastoconidia mixed with whole blood in a titration experiment yielded a linear PCR signal over a range of 3 orders of magnitude. The TaqMan-based PCR assay for C. albicans exhibited a low limit of detection (5 CFU/ml of blood) and an excellent reproducibility (96 to 99%). While the C. albicans species-specific probe had 100% specificity for C. albicans , all Candida genus-specific probes cross-reacted with other organisms likely to coinfect patients with C. albicans infections. On the basis of these data, we determined the C. albicans loads with a species-specific probe from 122 blood samples from 61 hematology or oncology patients with clinically proven or suspected systemic Candida infections. Eleven positive samples exhibited a wide range of C. albicans loads, extending from 5 to 100,475 CFU/ml of blood. The sensitivity and specificity of the present assay were 100 and 97%, respectively, compared with the results of blood culture. These data indicate that the TaqMan-based PCR assay for quantitation of C. albicans with a species-specific probe provides an attractive alternative for the identification and quantitation of C. albicans rDNA in pure cultures and blood samples. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1128/jcm.41.7.3293-3298.2003 |
| DOI: | 10.1128/JCM.41.7.3293-3298.2003 |
| الاتاحة: | http://dx.doi.org/10.1128/jcm.41.7.3293-3298.2003 https://journals.asm.org/doi/pdf/10.1128/JCM.41.7.3293-3298.2003 |
| Rights: | https://journals.asm.org/non-commercial-tdm-license |
| رقم الانضمام: | edsbas.A6C7920C |
| قاعدة البيانات: | BASE |
| DOI: | 10.1128/jcm.41.7.3293-3298.2003 |
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