Academic Journal
The bdbDC operon of Bacillus subtilis encodes thiol-disulfide oxidoreductases required for competence development
| العنوان: | The bdbDC operon of Bacillus subtilis encodes thiol-disulfide oxidoreductases required for competence development |
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| المؤلفون: | Meima, R, Eschevins, C, Fillinger, S, Bolhuis, A, Hamoen, L W, Dorenbos, R, Quax, W J, van Dijl, J M, Provvedi, R, Chen, I, Dubnau, D, Bron, S |
| المصدر: | Meima , R , Eschevins , C , Fillinger , S , Bolhuis , A , Hamoen , L W , Dorenbos , R , Quax , W J , van Dijl , J M , Provvedi , R , Chen , I , Dubnau , D & Bron , S 2002 , ' The bdbDC operon of Bacillus subtilis encodes thiol-disulfide oxidoreductases required for competence development ' , The Journal of Biological Chemistry , vol. 277 , no. 9 , pp. 6994-7001 . https://doi.org/10.1074/jbc.M111380200 |
| سنة النشر: | 2002 |
| المجموعة: | University of Groningen research database |
| مصطلحات موضوعية: | ESCHERICHIA-COLI, BOND FORMATION, TRANSCRIPTION FACTOR, MOLECULAR-CLONING, GENOME SEQUENCE, DNA-BINDING, PROTEIN, GENE, IDENTIFICATION, TRANSFORMATION |
| الوصف: | The development of genetic competence in the Gram-positive eubacterium Bacillus subtilis is a complex post-exponential process. Here we describe a new bicistronic operon, bdbDC, required for competence development, which was identified by the B. subtilis Systematic Gene Function Analysis program. Inactivation of either the bdbC or bdbD genes of this operon results in the loss of transformability without affecting recombination or the synthesis of ComK, the competence transcription factor. BdbC and BdbD are orthologs of enzymes known to be involved in extracytoplasmic disulfide bond formation. Consistent with this, BdbC and BdbD are needed for the secretion of the Escherichia coli disulfide bond-containing alkaline phosphatase, PhoA, by R subtilis. Similarly, the amount of the disulfide bond-containing competence protein ComGC is severely reduced in bdbC or bdbD mutants. In contrast, the amounts of the competence proteins ComGA and ComEA remain unaffected by bdbDC mutations. Taken together, these observations imply that in the absence of either BdbC or BdbD, ComGC is unstable and that BdbC and BdbD catalyze the formation of disulfide bonds that are essential for the DNA binding and uptake machinery. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | https://research.rug.nl/en/publications/b98f4483-f7f3-424d-8b83-78d5118180fb |
| DOI: | 10.1074/jbc.M111380200 |
| الاتاحة: | https://hdl.handle.net/11370/b98f4483-f7f3-424d-8b83-78d5118180fb https://research.rug.nl/en/publications/b98f4483-f7f3-424d-8b83-78d5118180fb https://doi.org/10.1074/jbc.M111380200 https://pure.rug.nl/ws/files/214573881/The_bdbDC_operon_of_Bacillus_subtilis_encodes_thiol_disulfide_oxidoreductases_required.pdf |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.A6B3810D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1074/jbc.M111380200 |
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