Academic Journal
Optimum extraction of polysaccharides from Opuntia dillenii and evaluation of its antioxidant activities
| العنوان: | Optimum extraction of polysaccharides from Opuntia dillenii and evaluation of its antioxidant activities |
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| المؤلفون: | Yang, Q., Chen, H., Zhou, X., Zhang, J. |
| سنة النشر: | 2013 |
| المجموعة: | National Research Council Canada: NRC Publications Archive |
| مصطلحات موضوعية: | Anti-oxidant activities, Antioxidant capacity, Box-Behnken design, Extraction conditions, Extraction temperatures, Extraction time, Opuntia dillenii, Response surface methodology, Agents, Optimization, Polysaccharides, Surface properties, Extraction, Opuntia |
| الوصف: | Response surface methodology (RSM) was applied to optimize the extraction of crude polysaccharides from Opuntia dillenii (Ker-Gaw) Haw. A three-level, four-variable Box-Behnken design was employed to obtain the best possible combination of extraction temperature (80-90°C), extraction time (50-70 min), number of extraction cycle (1-3 times), and ratio of water to raw material (8:1-12:1, v/w) for maximum yield of crude polysaccharide. Besides, the antioxidant capacity of crude polysaccharide was evaluated by DPPH assay. The results showed that optimized extraction conditions were extraction temperature 85°C, extraction time 63.7 min, extraction 2 times and ratio of water to raw material 11.14:1. Under these conditions, the experimental yield was 27.36 ± 0.21%, which is well in close agreement with the value (27.44%) predicted by RSM model. Pharmacological test showed that O. dillenii crude polysaccharides had a good antioxidant activity. © 2013 Elsevier Ltd. All rights reserved. ; Peer reviewed: Yes ; NRC publication: Yes |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | text |
| اللغة: | English |
| تدمد: | 0144-8617 |
| Relation: | Carbohydrate Polymers, Volume: 97, Issue: 2, Publication date: 2013, Pages: 736–742 |
| DOI: | 10.1016/j.carbpol.2013.05.057 |
| الاتاحة: | https://doi.org/10.1016/j.carbpol.2013.05.057 https://nrc-publications.canada.ca/eng/view/object/?id=8fc6d886-bbaf-4e31-ae5f-b82822ef77e7 https://nrc-publications.canada.ca/fra/voir/objet/?id=8fc6d886-bbaf-4e31-ae5f-b82822ef77e7 |
| رقم الانضمام: | edsbas.A69038C1 |
| قاعدة البيانات: | BASE |
| تدمد: | 01448617 |
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| DOI: | 10.1016/j.carbpol.2013.05.057 |