Academic Journal
The ubiquitin ligase HERC3 attenuates NF-κB-dependent transcription independently of its enzymatic activity by delivering the RelA subunit for degradation
| العنوان: | The ubiquitin ligase HERC3 attenuates NF-κB-dependent transcription independently of its enzymatic activity by delivering the RelA subunit for degradation |
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| المؤلفون: | Hochrainer, Karin, Pejanovic, Nadja, Olaseun, Victoria A., Zhang, Sheng, Iadecola, Costantino, Anrather, Josef |
| بيانات النشر: | Oxford University Press |
| سنة النشر: | 2015 |
| المجموعة: | ARCA - IGC Repository (Access to Research and Communication Annals: Instituto Gulbenkian de Ciência) |
| مصطلحات موضوعية: | HERC3 protein, human |
| الوصف: | Activation of NF-κB-dependent transcription represents an important hallmark of inflammation. While the acute inflammatory response is per se beneficial, it can become deleterious if its spatial and temporal profile is not tightly controlled. Classically, NF-κB activity is limited by cytoplasmic retention of the NF-κB dimer through binding to inhibitory IκB proteins. However, increasing evidence suggests that NF-κB activity can also be efficiently contained by direct ubiquitination of NF-κB subunits. Here, we identify the HECT-domain ubiquitin ligase HERC3 as novel negative regulator of NF-κB activity. We find that HERC3 restricts NF-κB nuclear import and DNA binding without affecting IκBα degradation. Instead HERC3 indirectly binds to the NF-κB RelA subunit after liberation from IκBα inhibitor leading to its ubiquitination and protein destabilization. Remarkably, the regulation of RelA activity by HERC3 is independent of its inherent ubiquitin ligase activity. Rather, we show that HERC3 and RelA are part of a multi-protein complex containing the proteasome as well as the ubiquitin-like protein ubiquilin-1 (UBQLN1). We present evidence that HERC3 and UBQLN1 provide a link between NF-κB RelA and the 26S proteasome, thereby facilitating RelA protein degradation. Our findings establish HERC3 as novel candidate regulating the inflammatory response initiated by NF-κB. ; American Heart Association Scientist Development: (SDG102600298), National Institute of Health Grants: (HL077308, NS34179), Funding for open access charge: NIH (NS34179). |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | http://nar.oxfordjournals.org/content/43/20/9889.long; Karin Hochrainer, Nadja Pejanovic, Victoria A. Olaseun, Sheng Zhang, Costantino Iadecola, and Josef Anrather The ubiquitin ligase HERC3 attenuates NF-κB-dependent transcription independently of its enzymatic activity by delivering the RelA subunit for degradation Nucl. Acids Res. (16 November 2015) 43 (20): 9889-9904 first published online October 17, 2015 doi:10.1093/nar/gkv1064; http://hdl.handle.net/10400.7/534 |
| DOI: | 10.1093/nar/gkv1064 |
| الاتاحة: | http://hdl.handle.net/10400.7/534 https://doi.org/10.1093/nar/gkv1064 |
| Rights: | openAccess ; http://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.A64D3748 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/nar/gkv1064 |
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