التفاصيل البيبلوغرافية
| العنوان: |
NbNBR1 does not affect the level of β C1 mRNA but increases βC1 accumulation through formation of cytoplasmic granules. |
| المؤلفون: |
Tingting Zhou (163678), Mingzhen Zhang (1735672), Pan Gong (4070329), Fangfang Li (1972918), Xueping Zhou (131154) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Medicine, Microbiology, Cell Biology, Molecular Biology, Ecology, Immunology, Cancer, Mental Health, Infectious Diseases, Plant Biology, Virology, Biological Sciences not elsewhere classified, targets cell organelles, form cytoplasmic granules, βc1 protein encoded, vacuolar degradation mechanism, suppressed βc1 accumulation, promoted virus infection, inhibited virus infection, div >< p, nicotiana benthamiana <, k4a , benthamiana <, n <, mediated degradation, dependent degradation, nbnbr1 <, viral satellite, viral roles, viral role |
| الوصف: |
(A) End point PCR analyses of βC1 expressions in the N . benthamiana leaves expressing βC1-YFP and Myc-NbNBR1+βC1-YFP. (B) BiFC assay was performed to investigate the interaction between NbNBR1 and βC1 at different time points. Images were captured under a confocal microscope at 36, 48 and 60 hpi, respectively. Bar = 10 μm. (C, D) Western blot assays were performed to determine the accumulations of NbNBR1 and βC1 using anti-HA antibodies (pEarlyGate201-YN vector used in this study including the HA tag) at various time points. The CBB-stained Rubisco large subunit gel was used to show equal sample loadings (C, D), respectively. (E) A quantification method was used to validate the size of NbNBR1-βC1 complex at different time points. Units: μm. (TIF) |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/NbNBR1_does_not_affect_the_level_of_i_C1_i_mRNA_but_increases_C1_accumulation_through_formation_of_cytoplasmic_granules_/16688570 |
| DOI: |
10.1371/journal.ppat.1009956.s004 |
| الاتاحة: |
https://doi.org/10.1371/journal.ppat.1009956.s004 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.A5D76363 |
| قاعدة البيانات: |
BASE |