Academic Journal
Intracellular pH response to weak acid stress in individual vegetative Bacillus subtilis cells
| العنوان: | Intracellular pH response to weak acid stress in individual vegetative Bacillus subtilis cells |
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| المؤلفون: | Pandey, Rachna, Vischer, Norbert OE, Smelt, Jan PPM, van Beilen, Johan WA, Ter Beek, Alexander, De Vos, Winnok, Brul, Stanley, Manders, Erik MM |
| المصدر: | APPLIED AND ENVIRONMENTAL MICROBIOLOGY ; ISSN: 0099-2240 ; ISSN: 1098-5336 |
| سنة النشر: | 2016 |
| المجموعة: | Ghent University Academic Bibliography |
| مصطلحات موضوعية: | Biology and Life Sciences, PROTON MOTIVE FORCE, MEMBRANE H+-ATPASE, SACCHAROMYCES-CEREVISIAE, ESCHERICHIA-COLI, ORGANIC-ACIDS, SORBIC ACID, GROWTH, YEAST, COMPARTMENT, HOMEOSTASIS |
| الوصف: | Intracellular pH (pH(i)) critically affects bacterial cell physiology. Hence, a variety of food preservation strategies are aimed at perturbing pHi homeostasis. Unfortunately, accurate pHi quantification with existing methods is suboptimal, since measurements are averages across populations of cells, not taking into account interindividual heterogeneity. Yet, physiological heterogeneity in isogenic populations is well known to be responsible for differences in growth and division kinetics of cells in response to external stressors. To assess in this context the behavior of intracellular acidity, we have developed a robust method to quantify pHi at single-cell levels in Bacillus subtilis. Bacilli spoil food, cause disease, and are well known for their ability to form highly stress-resistant spores. Using an improved version of the genetically encoded ratiometric pHluorin (IpHluorin), we have quantified pHi in individual B. subtilis cells, cultured at an external pH of 6.4, in the absence or presence of weak acid stresses. In the presence of 3 mM potassium sorbate, a decrease in pHi and an increase in the generation time of growing cells were observed. Similar effects were observed when cells were stressed with 25 mM potassium acetate. Time-resolved analysis of individual bacteria in growing colonies shows that after a transient pH decrease, long-term pH evolution is highly cell dependent. The heterogeneity at the single-cell level shows the existence of subpopulations that might be more resistant and contribute to population survival. Our approach contributes to an understanding of pHi regulation in individual bacteria and may help scrutinizing effects of existing and novel food preservation strategies. IMPORTANCE : This study shows how the physiological response to commonly used weak organic acid food preservatives, such as sorbic and acetic acids, can be measured at the single-cell level. These data are key to coupling often-observed single-cell heterogeneous growth behavior upon the addition of weak organic ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | https://biblio.ugent.be/publication/8525126; https://biblio.ugent.be/publication/8525126/file/8525131 |
| DOI: | 10.1128/aem.02063-16 |
| الاتاحة: | https://biblio.ugent.be/publication/8525126 http://hdl.handle.net/1854/LU-8525126 https://doi.org/10.1128/aem.02063-16 https://biblio.ugent.be/publication/8525126/file/8525131 |
| Rights: | No license (in copyright) ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.A4CD8126 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1128/aem.02063-16 |
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