Academic Journal
Broad complex and wing development in cockroaches
| العنوان: | Broad complex and wing development in cockroaches |
|---|---|
| المؤلفون: | Fernández-Nicolas, Ana, Ventós-Alfonso, Alba, Kamsoi, Orathai, Clark-Hachtel, Courtney, Tomoyasu, Yoshinori, Bellés, Xavier |
| المساهمون: | Agencia Estatal de Investigación (España), Generalitat de Catalunya, European Commission, Miami University, National Science Foundation (US) |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2022 |
| المجموعة: | Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council) |
| مصطلحات موضوعية: | Broad complex, Wing-related genes, Initiation of wing development, Blattella, Nubbin |
| الوصف: | In hemimetabolan insects, the transcription factor Broad complex (Br–C) promotes wing growth and development during the nymphal period. We wondered whether Br–C could trigger the initiation of wing development, using the cockroach Blattella germanica as a model. We show that first instar nymphs have their unique identity of these three thoracic segments specified. During embryogenesis, the expression of Br–C and some wing-related genes show two matching waves. The first takes place before the formation of the germ band, which might be involved in the establishment of various developmental fields including a potential “wing field”, and the second wave around organogenesis, possibly involved in the initiation of wing development. However, the expression of Br–C in early embryogenesis concentrates in the developing central nervous system, thus not co-localizing with the expression of the typical wing-related gene vestigial, which is expressed at the edge of the thoracic and abdominal segments. This suggests that Br–C is not specifically involved in the establishment of a potential “wing field” in early embryogenesis. Moreover, maternal RNAi for Br–C depletes the first wave of Br–C expression but does not affect the early expression of wing-related genes. As maternal Br–C RNAi did not deplete the second expression wave of Br–C, we could not evaluate if Br–C is involved in the initiation of wing development. Alternatively, using nymphal RNAi of Br–C and Sex combs reduced (Scr), we show that Br–C contributes to the formation of ectopic wing structures that develop in the prothorax when Scr is depleted. The gene most clearly influenced by Br–C RNAi is nubbin (nub), which, in nymphs is crucial for wing growth. Together, these results suggest that Br–C does not specifically contribute to the establishment of the “wing field”, but it does seem important later, in the initiation of wing development, enhancing the expression of wing-related genes, especially nub. This supports the hypothesis previously proposed by the ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0965-1748 |
| Relation: | #PLACEHOLDER_PARENT_METADATA_VALUE#; info:eu-repo/grantAgreement/AEI//PID2019-104483GB-I00; info:eu-repo/grantAgreement/MINECO//CGL2015-64727-P; info:eu-repo/grantAgreement/MINECO//CGL2012-36251; Publisher's version; https://doi.org/10.1016/j.ibmb.2022.103798; Sí; Insect Biochemistry and Molecular Biology 147: 103798 (2022); http://hdl.handle.net/10261/271992; http://dx.doi.org/10.13039/501100011033; http://dx.doi.org/10.13039/501100000780; http://dx.doi.org/10.13039/501100002809; http://dx.doi.org/10.13039/100000001 |
| DOI: | 10.1016/j.ibmb.2022.103798 |
| DOI: | 10.13039/501100011033 |
| DOI: | 10.13039/501100000780 |
| DOI: | 10.13039/501100002809 |
| DOI: | 10.13039/100000001 |
| الاتاحة: | http://hdl.handle.net/10261/271992 https://doi.org/10.1016/j.ibmb.2022.103798 https://doi.org/10.13039/501100011033 https://doi.org/10.13039/501100000780 https://doi.org/10.13039/501100002809 https://doi.org/10.13039/100000001 |
| Rights: | open |
| رقم الانضمام: | edsbas.A431474A |
| قاعدة البيانات: | BASE |
| تدمد: | 09651748 |
|---|---|
| DOI: | 10.1016/j.ibmb.2022.103798 |