Academic Journal
Single-Cell 5-Formylcytosine Landscapes of Mammalian Early Embryos and ESCs at Single-Base Resolution
| العنوان: | Single-Cell 5-Formylcytosine Landscapes of Mammalian Early Embryos and ESCs at Single-Base Resolution |
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| المؤلفون: | Zhu, Chenxu, Gao, Yun, Guo, Hongshan, Xia, Bo, Song, Jinghui, Wu, Xinglong, Zeng, Hu, Kee, Kehkooi, Tang, Fuchou, Yi, Chengqi |
| المساهمون: | Yi, CQ (reprint author), Peking Univ, Sch Life Sci, State Key Lab Prot & Plant Gene Res, Beijing 100871, Peoples R China., Tang, FC (reprint author), Peking Univ, Sch Life Sci, Biodynam Opt Imaging Ctr, Beijing 100871, Peoples R China., Tang, FC (reprint author), Peking Univ, Sch Life Sci, Beijing Adv Innovat Ctr Genom, Beijing 100871, Peoples R China., Tang, FC, Yi, CQ (reprint author), Peking Univ, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China., Tang, FC (reprint author), Peking Univ, Mini, Peking Univ, Sch Life Sci, State Key Lab Prot & Plant Gene Res, Beijing 100871, Peoples R China., Peking Univ, Sch Life Sci, Biodynam Opt Imaging Ctr, Beijing 100871, Peoples R China., Peking Univ, Sch Life Sci, Beijing Adv Innovat Ctr Genom, Beijing 100871, Peoples R China., Peking Univ, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China., Tsinghua Univ, Sch Med, Dept Basic Med Sci, Ctr Stem Cell Biol & Regenerat Med, Beijing 100084, Peoples R China., Peking Univ, Minist Educ, Key Lab Cell Proliferat & Differentiat, Beijing 100871, Peoples R China., Peking Univ, Coll Chem & Mol Engn, Dept Chem Biol, Beijing 100871, Peoples R China., Peking Univ, Coll Chem & Mol Engn, Synthet & Funct Biomol Ctr, Beijing 100871, Peoples R China., NYU, Sch Med, Sackler Inst Grad Biomed Sci, New York, NY 10016 USA., NYU, Sch Med, Inst Computat Med, New York, NY 10016 USA., Tang, FC (reprint author), Peking Univ, Minist Educ, Key Lab Cell Proliferat & Differentiat, Beijing 100871, Peoples R China., Yi, CQ (reprint author), Peking Univ, Coll Chem & Mol Engn, Dept Chem Biol, Beijing 100871, Peoples R China., Yi, CQ (reprint author), Peking Univ, Coll Chem & Mol Engn, Synthet & Funct Biomol Ctr, Beijing 100871, Peoples R China. |
| المصدر: | SCI |
| بيانات النشر: | CELL STEM CELL |
| سنة النشر: | 2017 |
| المجموعة: | Peking University Institutional Repository (PKU IR) / 北京大学机构知识库 |
| مصطلحات موضوعية: | MOUSE PREIMPLANTATION DEVELOPMENT, THYMINE DNA GLYCOSYLASE, GENOME-WIDE DETECTION, STEM-CELLS, CHROMATIN ACCESSIBILITY, SELF-RENEWAL, TET PROTEINS, REVEALS, 5-HYDROXYMETHYLCYTOSINE, 5-CARBOXYLCYTOSINE |
| الوصف: | Active DNA demethylation in mammals involves ten-eleven translocation (TET) family protein-mediated oxidation of 5-methylcytosine (5mC). However, base-resolution landscapes of 5-formylcytosine (5fC) (an oxidized derivative of 5mC) at the single-cell level remain unexplored. Here, we present "CLEVER-seq" (chemical-labeling-enabled C-to-T conversion sequencing), whichis a single-cell, single-base resolution 5fC-sequencing technology, based on biocompatible, selective chemical labeling of 5fC and subsequent C-to-T conversion during amplification and sequencing. CLEVER-seq shows intrinsic 5fC heterogeneity in mouse early embryos, Epi stem cells (EpiSCs), and embryonic stem cells (ESCs). CLEVER-seq of mouse early embryos also reveals the highly patterned genomic distribution and parental-specific dynamics of 5fC during mouse early pre-implantation development. Integrated analysis demonstrates that promoter 5fC production precedes the expression upregulation of a clear set of developmentally and metabolically critical genes. Collectively, our work reveals the dynamics of active DNA demethylation during mouse pre-implantation development and provides an important resource for further functional studies of epigenetic reprogramming in single cells. ; National Basic Research Program of China; National Natural Science Foundation of China [91519325, MOST2016YFC0900300, 21522201, 2014CB964900, 31271543, 81561138005]; Foundation for Innovative Research Groups of the National Natural Science Foundation of China [81521002] ; SCI(E) ; ARTICLE ; 5 ; 720-+ ; 20 |
| نوع الوثيقة: | journal/newspaper |
| اللغة: | English |
| تدمد: | 1934-5909 1875-9777 |
| Relation: | CELL STEM CELL.2017,20(5),720-+.; 1907758; http://hdl.handle.net/20.500.11897/473502; WOS:000400565700019 |
| DOI: | 10.1016/j.stem.2017.02.013 |
| الاتاحة: | https://hdl.handle.net/20.500.11897/473502 https://doi.org/10.1016/j.stem.2017.02.013 |
| رقم الانضمام: | edsbas.A42190D2 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 19345909 18759777 |
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| DOI: | 10.1016/j.stem.2017.02.013 |